Bruch membrane aging alters the gene expression profile of human retinal pigment epithelium
Bruch membrane aging alters the gene expression profile of human retinal pigment epithelium
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DOI：
10.1080/02713680500514628
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发表时间：
2006-02-01
影响因子：
2
通讯作者：
Del Priore, LV
中科院分区：
文献类型：
--
作者：
Cai, H;Del Priore, LV
我们研究了Bruch膜内与年龄相关的变化对RPE基因表达谱的影响。将永生化的人ARPE-19细胞接种到来自年轻和年长供体的无细胞人Bruch膜上，并在72小时后收获;分离总RNA，并使用Affytron Human Genome U95A基因芯片测定基因表达谱。12个基因上调，8个基因下调与布鲁赫膜老化; RT-PCR证实，布鲁赫膜老化上调基因编码的转化生长因子α和下调基因的玻连蛋白和膜转运ABCC 5的RPE细胞。这些变化在老年性疾病如黄斑变性的发病机制中的作用仍有待阐明。
We investigated the effects of age-related changes within the Bruch membrane on the gene expression profile of the RPE. Immortalized human ARPE-19 cells were seeded onto acellular human Bruch membrane from younger and older donors and harvested 72 hr later; total RNA was isolated and the gene expression profile was determined using the Affymetrix Human Genome U95A gene chip. Twelve genes were upregulated and 8 genes were downregulated with Bruch membrane aging; RT-PCR confirms that Bruch membrane aging upregulates genes in RPE cells encoding for transforming growth factor alpha and downregulates genes for vitronectin and the membrane transporter ABCC5. The role of these changes in the pathogenesis of age-related diseases such as macular degeneration remains to be elucidated.