Matrix methods for solving protein substructures of chlorine and sulfur from anomalous data
Matrix methods for solving protein substructures of chlorine and sulfur from anomalous data
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DOI：
10.1107/s0907444901016535
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发表时间：
2001-12-01
期刊：
影响因子：
--
通讯作者：
Abrahams, JP
中科院分区：
文献类型：
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作者：
de Graaff, RAG;Hilge, M;Abrahams, JP
由硫、磷和有序溶剂氯离子等自然存在的元素的反常散射(波长=1.54埃)获得的微弱信号被用来确定这些原子的原子位置。讨论了两个例子：四方蛋清溶菌酶的硫氯亚结构和一个含有10个P原子的寡核苷酸。在标准旋转阳极发生器上采集的CuKα辐射数据，对溶菌酶的亚结构进行了解析。这里给出的结果说明了矩阵方法的力量，这些方法将在直接方法包压缩的下一次分发中实现。
The weak signal obtained from the anomalous scattering (at lambda = 1.54 Angstrom) of naturally occurring elements such as sulfur, phosphorus and ordered solvent chloride ions is used to determine the atomic positions of these atoms. Two examples are discussed: the sulfur and chlorine substructure of tetragonal hen egg-white lysozyme and an oligonucleotide containing ten P atoms. The substructure of lysozyme was also solved from Cu K alpha radiation data collected on a standard rotating-anode generator. The results presented here are an illustration of the power of the matrix methods, which are to be implemented in next distribution of the direct methods package CRUNCH.