Physiology and molecular characteristics of a pine wilt nematode-trapping fungus, Monacrosporium megalosporum
Physiology and molecular characteristics of a pine wilt nematode-trapping fungus, Monacrosporium megalosporum
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DOI：
10.1007/s00284-004-4268-9
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发表时间：
2004-09-01
影响因子：
2.6
通讯作者：
Morinaga, T
中科院分区：
文献类型：
--
作者：
Kano, S;Aimi, T;Morinaga, T
摘要从自然界分离到一株捕获线虫的巨孢单孢子菌，对其形态、生理和分子特征进行了研究。这种真菌的线虫捕获装置是一个三维网络。这种真菌捕获松树枯萎线虫（Bursaphelenchus xylophilus），但不捕获形态类似于B. xylophilus的非植物致病性线虫。rDNA ITS区核苷酸序列的进化关系与M. thaumasium和Genicalifera eudermata相似，这两种植物也具有三维网络的线虫捕获装置。酸性pH抑制原生质体的释放和再生。pH值低于6.0时，原生质体细胞质出现肉芽化。在pH值低于4时，菌丝在琼脂培养基上的生长也受到抑制，但在pH值为9时则不受抑制。这些结果强烈表明，酸雨对该真菌的活性有抑制作用。因此，松材萎蔫病的发生可能是酸雨的次生效应。
We isolated the nematode-trapping fungus Monacrosporium megalosporum from nature and examined its morphology, physiology and molecular characteristics. The nematode-trapping device of this fungus is a three-dimensional network. This fungus captures the pine wilt nematode (Bursaphelenchus xylophilus), but not a non-phytopathogenic nematode that is morphologically similar to B. xylophilus. The pbylogenic relationship of the nucleotide sequence of the rDNA ITS region was close to those of M. thaumasium and Genicalifera eudermata, which also have nematode-trapping devices that are three-dimensional networks. Acidic pH inhibited both the liberation and regeneration of protoplasts. Moreover, cytoplasmic granulation of protoplasts was found below pH 6.0. Mycelial growth on agar media was also inhibited below pH 4, but not at pH 9. These results strongly suggest that the activity of this fungus is inhibited by acid rain in the field. Therefore, development of pine wilt disease might be a secondary effect of acid rain.