Simple and rapid quantification of vancomycin in serum, urine and peritoneal/pleural effusion via UHPLC-MS/MS applicable to personalized antibiotic dosing
Simple and rapid quantification of vancomycin in serum, urine and peritoneal/pleural effusion via UHPLC-MS/MS applicable to personalized antibiotic dosing
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DOI：
10.1016/j.jpba.2017.04.029
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发表时间：
2017-08-05
影响因子：
3.4
通讯作者：
Kaska, Milan
中科院分区：
文献类型：
--
作者：
Javorska, Lenka;Krcmova, Lenka Kujovska;Kaska, Milan
对危及生命的细菌感染的治疗管理主要基于最佳抗生素治疗。在特殊情况下，在血液和靶组织中获得正确的万古霉素剂量可能很复杂，例如，其中发生大量液体隔离和/或急性肾衰竭。采用电喷雾（ESI）正离子模式下操作的UHPLC-MS/MS方法测定血清、尿液和腹膜/胸腔积液中的万古霉素。样品预处理包括稀释和简单的蛋白沉淀，其中仅需要少量（50 μ L）血清、尿液或腹膜/胸腔积液。万古霉素的分离在Meteoric Core C18 BIO柱（100 × 4.6 mm，2.7 μ m）上进行，用0.1%甲酸水溶液和乙腈进行梯度洗脱。总分析时间为4.5 min。发现该方法在血清2-60 μ M（或0.5-10 μ M）、腹膜/胸腔积液0.27-10 μ M（或2-60 μ M）和尿液25-300 μ M范围内呈线性，足以测定患者样品中的万古霉素。该方法的精密度和准确度在89%~ 104%之间，精密度均小于8%，3种体液样品（血清、尿液和腹腔/胸腔积液）中万古霉素浓度的测定方法简便、快速、可靠。该方法应适用于临床（药代动力学-药效学）研究中的大样本系列。(C)2017爱思唯尔B. V.保留所有权利。
Management of the therapy of life-threatening bacterial infection is extremely based on an optimal antibiotic treatment. Achieving the correct vancomycin dosage in blood and target tissues can be complicated in special situations, e.g., where large fluid sequestration and/or acute renal failure occur.A UHPLC-MS/MS method operating in electrospray (ESI) positive ion mode was applied for the determination of vancomycin in serum, urine and peritoneal/pleural effusion. Sample pretreatment was composed of dilution and simple protein precipitation where only a small volume (50 mu L) of serum, urine or peritoneal/pleural effusion was required. The separation of vancomycin was performed on a Meteoric Core C18 BIO column (100 x 4.6 mm, 2.7 mu m) by gradient elution with 0.1% formic acid in water and acetonitrile. The total time of analysis was 4.5 min. The method was found to be linear in the range of 2-60 mu M (or 0.5-10 mu M) for serum, 0.27-10 mu M (or 2-60 mu M) for peritoneal/pleural effusion and 25-300 mu M for urine, which was adequate for the determination of vancomycin in patient samples. The intra- and inter-day precision was below 8% RSD, and accuracy was from 89 to 104%.The UHPLC/MS-MS method offers a fast and reliable approach to determine vancomycin concentrations in three different human body fluid samples (serum, urine and peritoneal/pleural effusion) with a simple sample pretreatment that was the same for all selected specimens. This method should be applicable to large sample series in clinical (pharmacokineticipharmacodynamic) studies. (C) 2017 Elsevier B.V. All rights reserved.