Intracellular effect of ultrashort electrical pulses
Intracellular effect of ultrashort electrical pulses
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DOI：
10.1002/bem.71
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发表时间：
2001-09-01
影响因子：
1.9
通讯作者：
Buescher, ES
中科院分区：
文献类型：
--
作者：
Schoenbach, KH;Beebe, SJ;Buescher, ES
生物细胞的简单电学模型预测，当电脉冲持续时间减少到亚微秒范围时，电场与原核和真核细胞亚结构相互作用的可能性增加。用电场强度达5.3 MV/m的电脉冲作用于人嗜酸性细胞，验证了这一假设的有效性。当3-5个持续60 ns的脉冲作用于人嗜酸性粒细胞时，细胞内颗粒被修饰，而不会永久破坏质膜。由于脉冲持续时间极短，尽管施加在电池上的功率水平很高，但热效应可以忽略不计。细胞内效应将传统的电穿孔扩展到细胞亚结构，并在细胞凋亡诱导、基因传递到细胞核或根据电脉冲条件改变细胞功能方面开辟了新的应用潜力。生物电磁学学报，2001,22(2):444 -448。(C) 2001 Wiley-Liss。公司。
A simple electrical model for biological cells predicts an increasing probability for electric field interactions with cell substructures of prokaryotic and eukaryotic cells when the electric pulse duration is reduced into the sub-microsecond range. The validity of this hypothesis was verified experimentally by applying electrical pulses with electric field intensities of up to 5.3 MV/m to human eosinophils in vitro. When 3-5 pulses of 60 ns duration were applied to human eosinophils, intracellular granules were modified without permanent disruption of the plasma membrane. In spite of the extreme electrical power levels applied to the cells thermal effects could be neglected because of the ultrashort pulse duration. The intracellular effect extends conventional electroporation to cellular substructures and opens the potential for new applications in apoptosis induction, gene delivery to the nucleus, or altered cell functions, depending on the electrical pulse conditions. Bioelectromagnetics 22:440-448, 2001. (C) 2001 Wiley-Liss. Inc.