Enhancement of mast cell differentiation in vitro by T cell factor(s).
Enhancement of mast cell differentiation in vitro by T cell factor(s).
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T 细胞因子在体外增强肥大细胞分化。
DOI：
10.1159/000232853
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Ishizaka,T
中科院分区：
文献类型：
--
作者：
Ginsburg,H;Olson,EC;Huff,TF;Okudaira,H;Ishizaka,T
将经马血清免疫的Balb/c小鼠淋巴细胞置于含马血清的胚成纤维细胞单层培养基中培养，获得肥大细胞集落。为了表征肥大细胞的前体，对免疫小鼠的肠系膜淋巴结细胞进行分离，获得非贴壁细胞、B细胞缺失部分和T细胞缺失部分；各组分在成纤维细胞单层上培养。肥大细胞集落从非贴壁细胞和B细胞缺失部分发育而不是从T细胞缺失部分发育。然而，如果加入从马血清T细胞培养中获得的无细胞上清，同样的T细胞缺失部分的培养物会形成肥大细胞集落。当同样的T细胞在马无血清培养基中孵育时，没有获得促进肥大细胞生长的可溶性因子。淋巴结中大多数肥大细胞前体是非粘附细胞，既不含免疫球蛋白，也不含th1抗原。结果还表明，抗原刺激的T细胞释放的可溶性因子促进了成熟肥大细胞的前体分化。
Mast cell colonies were obtained when lymph node cells of horse serum-immunized Balb/c mice were cultured in a horse serum-containing medium on embryonic fibroblast monolayer. In order to characterize precursors of mast cells, mesenteric lymph node cells from the immunized mice were fractionated to obtain nonadherent cells, a B cell-depleted fraction and a T cell-depleted fraction; and each fraction was cultured on fibroblast monolayer. Mast cell colonies developed from nonadherent cells and from the B cell-depleted fraction but not from the T cell-depleted fraction. However, cultures of the same T cell-depleted fraction developed mast cell colonies if cell-free supernatant obtained from culture of horse serum-primed T cells was added. Soluble factors promoting mast cell growth were not obtained when the same T cells were incubated in horse serum-free medium. It appears that the majority of mast cell precursors in the lymph nodes are nonadherent cells and bear neither immunoglobulin nor Thy 1 antigen. The results also suggested that soluble factor(s) released from antigen-stimulated T cells enhanced the differentiation of the precursors to mature mast cells.