Adhesion of Shewanella oneidensis MR-1 to Goethite: A Two-Dimensional Correlation Spectroscopic Study
Adhesion of Shewanella oneidensis MR-1 to Goethite: A Two-Dimensional Correlation Spectroscopic Study
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Shewanella oneidensis MR-1 对针铁矿的粘附：二维相关光谱研究
DOI：
10.1021/acs.est.6b00066
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发表时间：
2016
影响因子：
11.4
通讯作者：
Jing CY
中科院分区：
文献类型：
--
作者：
Yan Wei;Jing Chuanyong;Wang Hongbo;Jing CY
细菌在矿物表面的粘附是一个重要但未被充分认识的过程。为了揭示这一分子水平的过程和机制，采用流动池衰减全反射（ATR）傅里叶变换红外（FTIR）光谱结合二维相关光谱（2D-COS）技术研究了希瓦氏菌（Shewanella oneidensis MR-1）细胞与针铁矿的粘附过程. FTIR结果表明，细菌磷酸盐在单齿和双齿内球复合物的形成中起重要作用，而细胞表面的羧基对其粘附作用较小。短期二维COS分析（0-120 min）和长期（2-18 h）阶段的研究表明，粘附过程按以下顺序进行：细胞表面蛋白质氢键的变化>单齿内球表面复合物的形成>外球表面复合物的形成>细胞表面蛋白质二级结构的转化>形成额外的桥接双齿表面络合物。此外，MR-1细胞在针铁矿上的粘附是pH依赖性的，这是由于pH对细胞结构和界面电荷的影响。原位ATR-FTIR结合2D-COS分析突出了其在探索涉及微生物的复杂表面反应方面的巨大潜力。这些结果提高了我们在分子水平上对微生物-矿物相互作用的理解，并对一系列生物地球化学过程具有重要意义。
Bacterial adhesion to mineral surfaces is an important but underappreciated process. To decipher the molecular level process and mechanism, the adhesion ofShewanella oneidensisMR-1 cells to goethite was investigated using flow-cell attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy coupled with two-dimensional correlation spectroscopy (2D-COS) analysis. The FTIR results indicate that bacterial phosphate-moieties play an important role in the formation of mono- and bidentate inner-sphere complexes, whereas carboxylic groups on cell surface only have a minor contribution to its adhesion. The 2D-COS analysis in short-term (0–120 min) and long-term (2–18 h) stages reveal that the adhesion process was in the following sequence: change in H-bonds of proteins on cell surfaces > formation of monodentate inner-sphere surface complexes > formation of outer-sphere surface complexes > transformation of protein secondary structure on cell surfaces > formation of additional bridging bidentate surface complexes. In addition, the adhesion of MR-1 cells on goethite was pH dependent due to pH impacts on the cell structure and the interface charge. The in situ ATR-FTIR integrated with 2D-COS analysis highlights its great potential in exploring complex surface reactions with microbes involved. These results improve our understanding of microbe–mineral interactions at the molecular level and have significant implications for a series of biogeochemical processes.