Involvement of Sl00–related Calcium–binding Protein pEL98 (or mts1) in Cell Motility and Tumor Cell Invasion
Involvement of Sl00–related Calcium–binding Protein pEL98 (or mts1) in Cell Motility and Tumor Cell Invasion
复制标题
Sl00 相关钙结合蛋白 pEL98（或 mts1）参与细胞运动和肿瘤细胞侵袭
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
S. Sakiyama
中科院分区：
文献类型：
--
作者：
K. Takenaga;Yohko Nakamura;H. Endo;S. Sakiyama
我们研究了在各种正常和转化细胞中细胞运动与pEL 9 S（mtsl）mRNA和蛋白表达的关系。pEL 98（mtsl）在v-Ha-ras转化的NIH 3 T3细胞和v-Ha-ras或v-src转化的正常大鼠肾细胞中的表达在mRNA和蛋白水平上均高于相应的亲本细胞。在v-Ha-ras转化的正常大鼠成纤维细胞（3 Y1）中的表达也较3 Y1细胞中的表达增加。而v-src转化的3 Y1细胞中，有一个克隆（src 3 Y1-K）pEL 98（mtsl）的表达增加，而另一个克隆（src 3 Y1-H）pEL 98（mtsl）的表达降低。pEL 98（mtsl）的表达水平与细胞运动性相关，这是通过测量吞噬运动的细胞轨迹来检查的。为了测试pEL 98（mtsl）蛋白在细胞运动性中的直接参与，用pEL 98 cDNA转染显示低细胞运动性的src 3 Y1-H细胞。表达大量pEL 98蛋白的转染子显示出比src 3 Y1-H细胞显著更高的细胞运动性。在刘易斯肺癌的各种克隆中，pEL 98（mtsl）的表达也被发现与运动和侵袭能力相关。这些结果表明，pEL 98（mtsl）蛋白在调节细胞运动性和肿瘤细胞侵袭性中起作用。
We examined the relationship between cell motility and the expressions of pEL9S (mtsl) mRNA and protein in various imirine normal and transformed cells. The expression of pEL98 (mtsl) in v–Ha–ras–transformed NIH3T3 cells and in normal rat kidney cells transformed by either v–Ha–ras or v–src was increased over that in the corresponding parental cells at both mRNA and protein levels. The expression in normal rat fibroblasts (3Y1) transformed by v–Ha–ras was also increased compared with that in 3Y1 cells. However, the expression of pEL98 (mtsl) in 3Y1 cells transformed by v–src was increased in one clone (src 3Y1–K), but decreased in another clone (src 3Y1–H). The expression level of pEL98 (mtsl) correlated well with cell motility, which was examined by measuring cell tracks by phagokinesis. In order to test direct involvement of the pEL98 (mtsl) protein in cell motility, src 3Y1–H cells that showed low cell motility were transfected with pEL98 cDNA. The transfectants expressing large amounts of the pEL98 protein showed significantly higher cell motility than src 3Y1–H cells. The expression of pEL98 (mtsl) was also found to be correlated with motile and invasive abilities in various clones derived from Lewis lung carcinoma. These results suggest that the pEL98 (mtsl) protein plays a role in regulating cell motility and tumor cell invasiveness.
影响因子：
11.2
作者：
J. Kantor;Beth A. McCormick;Patricia S. Steeg;Bruce R. Zetter
通讯作者：
J. Kantor;Beth A. McCormick;Patricia S. Steeg;Bruce R. Zetter
G 蛋白参与基质介导的高和低实验转移性 B16 黑色素瘤克隆的运动和侵袭。
影响因子：
11.2
作者：
Lester,BR;McCarthy,JB;Sun,ZQ;Smith,RS;Furcht,LT;Spiegel,AM
通讯作者：
Spiegel,AM