Molecular analysis of the CALM/AF10 fusion: identical rearrangements in acute myeloid leukemia, acute lymphoblastic leukemia and malignant lymphoma patients
Molecular analysis of the CALM/AF10 fusion: identical rearrangements in acute myeloid leukemia, acute lymphoblastic leukemia and malignant lymphoma patients
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DOI：
10.1038/sj.leu.2401614
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发表时间：
2000-01-01
期刊：
影响因子：
11.4
通讯作者：
Dreyling, MH
中科院分区：
文献类型：
--
作者：
Bohlander, SK;Muschinsky, V;Dreyling, MH
在急性髓系白血病(AML)和急性淋巴细胞白血病(ALL)中发现的反复易位t(10；11)(p13；q14)导致可能的转录因子AF10融合，从而编码一种网状蛋白组装蛋白。以往的研究主要用荧光原位杂交(FISH)分析，发现在MO和M1亚型的未成熟急性髓系白血病(AML)和T细胞ALL中存在Calm/AF10重排。在这项研究中，我们分析了三名AML患者、一名T-ALL患者和两名先证者T淋巴母细胞淋巴瘤患者的Calm/AF10和AF10/Calm融合mRNAs。在所有6例患者中，CALET基因的断裂点都在编码区的3‘端(nt1926/1927或nt2091/2092)。在AF10中可以识别三个断点(NT 588/589、NT 882/883和NT 978/979)。这些数据表明，在患者身上发现的CAMAL/AF10融合只与AF10存在的部分略有不同，而且在AML患者中发现的融合与在淋巴系统恶性肿瘤患者中发现的没有明显差异。
The recurring translocation t(10;11)(p13;q14) which is found in acute myeloid leukemia (AML) and in acute lymphoblastic leukemia (ALL) results in the fusion of the putative transcription factor AF10 to CALM encoding a clathrin assembly protein. Previous studies using mainly fluorescence in situ hybridization (FISH) analysis have shown that the CALM/AF10 rearrangement is found in immature acute myeloid leukemia (AML) of subtype MO and M1 and in T cell ALL. In this study we analyzed the CALM/AF10 and AF10/CALM fusion mRNAs in a series of three patients with AML, one patient with T-ALL and two patients with precusor T lymphoblastic lymphoma. In all six patients the breakpoint in CALM is at the 3' end of the coding region (nt1926/1927 or nt 2091/2092). Three breakpoints could be identified in AF10 (nt 588/589, nt 882/883 and nt 978/979). These data demonstrate that the CALM/AF10 fusions found in patients differ only slightly with respect to the portion of AF10 present and that there is no obvious difference between the fusions found in AML patients compared to those found in patients with lymphoid malignancies.