Chlamydial infections in duck farms associated with human cases of psittacosis in France
Chlamydial infections in duck farms associated with human cases of psittacosis in France
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DOI：
10.1016/j.vetmic.2008.09.048
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发表时间：
2009-03-16
影响因子：
3.3
通讯作者：
Sachse, Konrad
中科院分区：
文献类型：
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作者：
Laroucau, Karine;de Barbeyrac, Bertille;Sachse, Konrad
2006 年 1 月至 3 月期间，法国通报了 5 例与鸭场相关的严重鹦鹉热病例。诊断检查包括对呼吸道样本进行血清学和/或 PCR 分子检测。因此，我们调查了发生人类感染的三个农场中的所有鸭群 (n = 11)。虽然所有样品的补体结合试验血清学均为阴性，但所有三个单位均通过 PCR 检测到泄殖腔和/或气管衣原体排泄物。值得注意的是，在 3 个受影响农场中的 2 个中，有 1 个鸭群检测呈强阳性，并且从两个农场的泄殖腔和/或气管拭子样本中分离出鹦鹉热衣原体 (C.) 菌株。人类样本和鸭分离物表现出相同的 PCR-RFLP 限制模式，这似乎是基因型 A 和 B 之间的中间体。对 ompA 基因序列的分析以及与模式菌株的比较表明，分离物不能被严格分配给鹦鹉热鹦鹉热的任何普遍接受的基因型。 MLVA 对 PCR 阳性人类样本的进一步分析揭示了两种不同的模式，这与之前分离的鹦鹉热鹦鹉鸭品系有关。 (C) 2008 Elsevier B.V. 保留所有权利。
Five severe cases of psittacosis in individuals associated with duck farms were notified in France between January and March 2006. Diagnostic examination included serology and/or molecular detection by PCR from respiratory samples.As a consequence, we investigated all duck flocks (n = 11) that were housed in the three farms where human infections occurred. While serology by complement fixation test was negative for all samples, cloacal and/or tracheal chlamydial excretion was detected by PCR in all three units. Notably, one duck flock was tested strongly positive in 2 of the 3 affected farms, and Chlamydophila (C.) psittaci strains were isolated from cloacal and/or tracheal swab samples from both farms.Human samples and duck isolates exhibited the same PCR-RFLP restriction pattern, which appeared to be an intermediate between genotypes A and B. Analysis of ompA gene sequences and comparison to those of the type strains showed that the isolates could not be strictly assigned to any of the generally accepted genotypes of C. psittaci. Further analysis by MLVA of the PCR-positive human samples revealed two distinct patterns, which were related to previously isolated C. psittaci duck strains. (C) 2008 Elsevier B.V. All rights reserved.