Periostin is an extracellular matrix protein required for eruption of incisors in mice
Periostin is an extracellular matrix protein required for eruption of incisors in mice
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DOI：
10.1016/j.bbrc.2006.02.016
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发表时间：
2006-04-14
影响因子：
3.1
通讯作者：
Kudo, A
中科院分区：
文献类型：
--
作者：
Kii, I;Amizuka, N;Kudo, A
作为啮齿动物特有的牙齿，门牙在一生中通过牙本质和牙釉质的不断形成而不断生长。切牙的持续萌出伴随着剪切带的形成，在剪切带中牙周膜被重塑。尽管剪切带在重塑中发挥了作用，但其分子生物学方面却鲜为人知。在这里，我们证明了Periostin对于剪切带的形成是必不可少的。Periostin(-/-)小鼠表现出门牙萌出障碍。组织学观察显示，Periostin的缺失导致剪切带的消失。电子显微镜显示，剪切带的消失是由于Periostin(-/-)小鼠未能消化胶原纤维所致。此外，使用抗Periostin抗体的免疫组织化学分析表明Periostin蛋白在剪切区的定位有限。Periostin是一种细胞外基质蛋白，免疫电子显微镜显示Periostin在体内与胶原纤维紧密结合。这些结果表明，Periostin在剪切带胶原基质的重塑中起作用。(C)2006 Elsevier Inc.，保留所有权利。
A characteristic tooth of rodents, the incisor continuously grows throughout life by the constant formation of dentin and enamel. Continuous eruption of the incisor is accompanied with formation of shear zone, in which the periodontal ligament is remodeled. Although the shear zone plays a role in the remodeling, its molecular biological aspect is barely understood. Here, we show that periostin is essential for formation of the shear zone. Periostin(-/-) mice showed an eruption disturbance of incisors. Histological observation revealed that deletion of periostin led to disappearance of the shear zone. Electron microscopy revealed that the disappearance of the shear zone resulted from a failure in digestion of collagen fibers in the periostin(-/-) mice. Furthermore, immunohistochemical analysis using anti-periostin antibodies demonstrated the restricted localization of periostin protein in the shear zone. Periostin is an extracellular matrix protein, and immunoelectron microscopy showed a close association of periostin with collagen fibrils in vivo. These results suggest that periostin functions in the remodeling of collagen matrix in the shear zone. (c) 2006 Elsevier Inc, All rights reserved.