CONGENITAL HYDROCEPHALUS - A NEW EXPERIMENTAL-MODEL WITH HISTOPATHOLOGICAL STUDY
CONGENITAL HYDROCEPHALUS - A NEW EXPERIMENTAL-MODEL WITH HISTOPATHOLOGICAL STUDY
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DOI：
10.1007/bf02337439
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发表时间：
1990-12-01
期刊：
影响因子：
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通讯作者：
PENTIMALLI, LCR
中科院分区：
文献类型：
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作者：
DITRAPANI, G;GARZETTI, GG;PENTIMALLI, LCR
我们描述了一种新的羔羊胎儿脑积水实验模型。对14只妊娠100-120天的绵羊进行手术，在Sylvius胎儿导水管内插入导管，阻断脑脊液（CSF）的流动。术后每日从导管远端测量颅内压（ICP）。超声记录心室扩张的进展情况。在ICP 100 mm/H2O下杀死动物，对胎儿进行尸检。神经病理检查显示脑室大面积扩张。室管膜细胞扁平，细胞内膜破裂。假性囊肿的生长、细胞分层和室旁生发细胞的增殖也被观察到。通过我们的新实验模型，我们能够控制ICP的上升，并将解剖损伤的演变与高ICP持续时间和开始时的胎龄联系起来。我们的模型也可以用于妊娠早期逆转脑积水的发展。因此，它可能为胎儿脑积水手术的适宜性提供信息。
We describe a new experimental model of fetal hydrocephalus in the lamb. 14 sheep were operated on at 100-120 days gestation for the insertion of a catheter into the fetal aqueduct of Sylvius to block cerebrospinal fluid (CSF) flow. After the operation the intracranial pressure (ICP) was measured daily from the distal end of the catheter. The progress of ventricular dilatation was recorded by ultrasound. At ICP 100 mm/H2O the animals were killed for postmortem examination of the fetuses. Neuropathological examination showed massive dilatation of the ventricles. The ependymal cells appeared to be flat and the cellular lining disrupted.Growth of pseudocysts, cellular stratification and proliferation of the paraventricular germinal cells were observed also. With our new experimental model we were able to control the rise in ICP and correlate the evolution of the anatomical damage with the duration of high ICP and with the gestational age at which it began. Our model can also be used at early stages of gestation for reversing the development of hydrocephalus. It might therefore provide information on the suitability of fetal hydrocephlus surgery.