Polymorphisms and the antiviral property of porcine Mx1 protein
Polymorphisms and the antiviral property of porcine Mx1 protein
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DOI：
10.1292/jvms.64.1085
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发表时间：
2002-12-01
影响因子：
1.2
通讯作者：
Watanabe, T
中科院分区：
文献类型：
--
作者：
Asano, A;Ko, JH;Watanabe, T
我们确定了I型干扰素诱导蛋白的cDNA序列，即来自PK（15）和LLC-PK 1细胞的猪Mx 1，并比较了两种Mx蛋白的抗病毒活性，包括Mx 1对水泡性口炎病毒（VSV）的多态性。PK（15）细胞来源的Mv 1 cDNA在编码区3'端有11 bp的缺失，与LLC-PK 1细胞来源的Mv 1 cDNA相比，Mv 1 cDNA编码8个氨基酸的替换和23个氨基酸的延伸。在表达Mx 1 mRNA的3 T3细胞中，VSV的复制受到抑制。然而，这种抑制的效率在表达来自PK和LLC的Mx 1 mPNA的细胞之间没有差异。这些结果表明猪Mx 1蛋白赋予对VSV的抗性。
We determined the cDNA sequences of the type I interferon-inducible proteins, pig Mx1 from PK(15) and LLC-PK1 cells, and compared the antiviral activities of both Mx proteins, including Mx1 polymorphisms against vesicular stomatitis virus (VSV). Mv1 cDNA derived from PK( 15) cells had an 11 bp-deletion in the 3' end of the coding region, and was estimated to encode 8 amino acid substitutions and a 23 amino acid extension compared to that from LLC-PK1 cells. VSV replication was inhibited in the 3T3 cells expressing Mx1 mRNA after the cDNA was transfected. However, the efficiency of this inhibition was not different between the cells expressing Mx1 mPNA from both PK and LLC, These results indicate that pig Mx1 protein confers resistance to VSV.