Export of infectious particles by Escherichia coli transfected with the RF DNA of Pf1, a virus of Pseudomonas aeruginosa strain K.
Export of infectious particles by Escherichia coli transfected with the RF DNA of Pf1, a virus of Pseudomonas aeruginosa strain K.
复制标题
转染 Pf1（铜绿假单胞菌 K 株病毒）的 RF DNA 的大肠杆菌输出感染性颗粒。
DOI：
10.1111/j.1365-2958.1991.tb01973.x
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发表时间：
1991
影响因子：
3.6
通讯作者：
Day,LA
中科院分区：
文献类型：
--
作者：
Kostrikis,LG;Reisberg,SA;Simon,MN;Wall,JS;Day,LA
Pf1是一种丝状单链DNA病毒，以铜绿假单胞菌（菌株K）为宿主。它是丝状细菌病毒中最长的，并且其中的DNA具有已知的最长的构象。Pfl病毒不能感染大肠杆菌（菌株MM 294）细胞，但当这些细胞用双链复制形式的Pt1 DNA（RF DNA，7.35 kb）转染时，它们输出低水平的感染性颗粒，这些颗粒在P.几种不同的结构物种，其中至少两种是传染性的，被输出。其中一种称为Epf 1，与Pf1的结构几乎相同，但E.大肠杆菌数比P.结果表明，宿主因素不仅影响病毒组装和输出的效率，而且还影响输出物种的实际结构。
Pf1 is a filamentous, single‐stranded DNA virus that hasPseudomas aeruginosa(strain K) as host. It is the longest of the filamentous bacterial viruses, and the DNA within it has the most extended conformation known. Pfl virus cannot infectEscherichia coli(strain MM294) cells, but when these cells are transfected with the double‐stranded replicative form of Pt1 DNA (RF DNA, 7.35 kb), they export low levels of infectious particles that create plaques on lawns ofP. aeruginosa.Several different structural species, at least two of which are infectious, are exported. One of them, called Epf1, has virtually the same structure as Pf1, but the amount of Epf1 exported byE. coliis 104lower than the amount of Pf1 exported byP. aeruginosa.The results imply that host factors affect not only the efficiency of virus assembly and export, but also the actual structures of the species exported.