First evidence of the Hepatitis E virus in environmental waters in Colombia.
First evidence of the Hepatitis E virus in environmental waters in Colombia.
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DOI：
10.1371/journal.pone.0177525
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Navas MC
中科院分区：
文献类型：
--
作者：
Baez PA;Lopez MC;Duque-Jaramillo A;Pelaez D;Molina F;Navas MC
戊型肝炎病毒（HEV）是引起肠道传播的急性病毒性肝炎的主要病原之一。在欧洲和亚洲的几个国家的环境样本中检测到HEV，构成了水传播感染的风险因素。在哥伦比亚，已在从患者和猪身上获得的样本中鉴定出戊型肝炎病毒，但尚未进行环境研究。为了确定HEV是否存在于环境沃茨中，在2012年12月至2014年4月期间收集了来自安蒂奥基亚州（哥伦比亚西北部）8个城市和2个村庄的饮用水厂和废水系统的主要来源的样本。用RT-PCR方法检测了30份主要水源水厂和16.7%（5/30）污水中的HEV基因组，检出率为23.3%（7/30）。从三个阳性污水样品中获得的病毒浓缩物用于培养HepG 2细胞培养物，并跟踪一个月;然而，在任何细胞培养物中均未检测到病毒基因组。这项研究表明，在安蒂奥基亚州，哥伦比亚的饮用水厂和废水的来源，HEV的循环。HEV在环境沃茨中的存在可能是该人群中水媒传播的风险。本研究的结果，以及在哥伦比亚的人类和猪的HEV循环的证据，应考虑由公共卫生当局的监测计划的发展和在该国的病毒性肝炎的指南中包括HEV感染诊断。这是哥伦比亚环境样品中HEV的第一份报告，也是拉丁美洲的第二份报告。
Hepatitis E virus (HEV) is one of the main causes of acute viral hepatitis of enteric transmission. HEV has been detected in environmental samples in several countries from Europe and Asia, constituting a risk factor for waterborne infection. In Colombia, HEV has been identified in samples obtained from patients as well as from swine, but no environmental studies have been carried out. To determine if HEV is present in environmental waters, samples from the main source of drinking water plant and of wastewater system of eight municipalities and two villages of Antioquia state (North West Colombia), were collected between December 2012 and April 2014. The HEV genome was detected by RT-PCR in 23.3% (7/30) of the samples from the main source of drinking water plants and in 16.7% (5/30) from sewage. Viral concentrates obtained from three positive sewage samples were used to inoculate HepG2 cell cultures that were followed for one month; however, the viral genome was not detected in any cell culture. This study demonstrates the circulation of HEV in both source of drinking water plants and wastewater in Antioquia state, Colombia. The presence of HEV in environmental waters could be a risk for waterborne transmission in this population. The findings of the present study, together with the evidence of HEV circulation in human and swine in Colombia, should be consider by public health authorities for the development of surveillance programs and the inclusion of HEV infection diagnosis in the guidelines of viral hepatitis in the country. This is the first report of HEV in environmental samples in Colombia and the second one in Latin America.