Effects of β-hydroxy-β-methylbutyrate free acid and cold water immersion on expression of CR3 and MIP-1β following resistance exercise
Effects of β-hydroxy-β-methylbutyrate free acid and cold water immersion on expression of CR3 and MIP-1β following resistance exercise
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DOI：
10.1152/ajpregu.00542.2013
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发表时间：
2014-04-01
影响因子：
2.8
通讯作者：
Hoffman, Jay R.
中科院分区：
文献类型：
--
作者：
Gonzalez, Adam M.;Fragala, Maren S.;Hoffman, Jay R.
β-羟基-β-甲基丁酸游离酸和冷水浸泡对抗阻运动后 CR3 和 MIP-1 β 表达的影响。 Am J Physiol Regul Integr Comp Physiol 306：R483-R489，2014 年。首次发表于 2014 年 2 月 5 日； doi：10.1152/ajpregu。 00542.2013.-对肌肉损伤运动的炎症反应需要单核细胞的动员和粘附。 3 型补体受体 (CR3) 和巨噬细胞炎症蛋白 (MIP)-1 beta 能够使单核细胞募集、粘附并随后渗透到受损的肌肉组织中。本研究的目的是检查冷水浸泡 (CWI) 和/或 β-羟基-β-甲基丁酸游离酸 (HMB-FA) 对 CR3 表达和 MIP-1 β 浓度的影响，在 70-80% 1 次重复最大值下进行四组最多 10 次的深蹲、硬拉和分腿深蹲练习后。 39 名接受过阻力训练的男性（22.2 +/- 2.5 岁）被随机分为四组：1) 安慰剂 (PL)、2) HMB-FA、3) HMB-FA-CWI 和 4) PL-CWI。 HMB-FA组每天摄入3g，CWI组运动后浸入10-12℃水中10分钟。在基线 (PRE)、运动后立即 (IP)、运动后 30 分钟 (30P)、运动后 24 小时 (24P) 和运动后 48 小时 (48P) 采集血液样本。通过流式细胞术测定循环 MIP-1 β 并测量 CD14+ 单核细胞上的 CR3 表达。未经治疗，与其他时间点相比，30P 时 CR3 表达显着升高（P = 0.030-0.047）。 HMB-FA 显着提高了 IP 和 24P 之间（P = 0.046）以及 IP 和 48P 之间（P = 0.046）表达 CR3 的单核细胞百分比。没有观察到 MIP-1 β 浓度的时间效应。恢复方式显示可以减弱运动后 CR3 的升高。此外，补充 HMB-FA 显着提高了恢复期间表达 CR3 的单核细胞的百分比。尽管炎症反应最有益的时间进程仍有待确定，但恢复方式可能会改变组织恢复期间免疫细胞的动员和粘附机制。
Effects of beta-hydroxy-beta-methylbutyrate free acid and cold water immersion on expression of CR3 and MIP-1 beta following resistance exercise. Am J Physiol Regul Integr Comp Physiol 306: R483-R489, 2014. First published February 5, 2014; doi: 10.1152/ajpregu. 00542.2013.-The inflammatory response to muscle-damaging exercise requires monocyte mobilization and adhesion. Complement receptor type 3 (CR3) and macrophage inflammatory protein (MIP)-1 beta enables monocyte recruitment, adhesion, and subsequent infiltration into damaged muscle tissue. The purpose of this study was to examine the effects of cold water immersion (CWI) and/or beta-hydroxy-beta-methylbutyrate free acid (HMB-FA) on CR3 expression and MIP-1 beta concentration after four sets of up to 10 repetitions of squat, dead lift, and split squat exercises at 70-80% 1-repetition maximum. Thirty-nine resistance-trained men (22.2 +/- 2.5 yr) were randomly divided into four groups: 1) placebo (PL), 2) HMB-FA, 3) HMB-FA-CWI, and 4) PL-CWI. The HMB-FA groups ingested 3 g/day, and CWI groups were submersed into 10-12 degrees C water for 10 min after exercise. Blood was sampled at baseline (PRE), immediately post-(IP), 30 min post-(30P), 24 h post-(24P), and 48 h post (48P)-exercise. Circulating MIP-1 beta was assayed and CR3 expression on CD14+ monocytes was measured by flow cytometry. Without treatment, CR3 expression significantly elevated at 30P compared with other time points (P = 0.030-0.047). HMB-FA significantly elevated the percentage of monocytes expressing CR3 between IP and 24P (P = 0.046) and between IP and 48P (P = 0.046). No time effect was observed for MIP-1 beta concentration. The recovery modalities showed to attenuate the rise in CR3 following exercise. Additionally, supplementation with HMB-FA significantly elevated the percentage of monocytes expressing CR3 during recovery. Although the time course that inflammatory responses are most beneficial remains to be determined, recovery modalities may alter immune cell mobilization and adhesion mechanisms during tissue recovery.