Hypertonic saline reduces lipopolysaccharide-induced mouse brain edema through inhibiting aquaporin 4 expression.
Hypertonic saline reduces lipopolysaccharide-induced mouse brain edema through inhibiting aquaporin 4 expression.
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DOI：
10.1186/cc11670
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发表时间：
2012-10-04
期刊：
影响因子：
--
通讯作者：
Yue S
中科院分区：
文献类型：
--
作者：
Cao C;Yu X;Liao Z;Zhu N;Huo H;Wang M;Ji G;She H;Luo Z;Yue S
3%氯化钠(NaC L)可减轻细菌性脑膜炎的脑水肿，抑制脑水通道蛋白4(AQP4)的表达。脂多糖(LPS)是大肠杆菌的主要致病成分。本研究旨在探讨3%氯化钠对内毒素诱导的小鼠脑水肿的影响，并阐明其可能的作用机制。用3%氯化钠对脂多糖诱导的小鼠脑水肿进行体内治疗。检测脑组织含水量、白介素1β、肿瘤坏死因子α、免疫球蛋白G、水通道蛋白4mRNA和蛋白的表达。用IL-1、β、3%氯化钠和蛋白激酶C特异性抑制剂Calphostin C处理原代星形胶质细胞。检测星形胶质细胞中AQP4mRNA和蛋白的表达。用单因素方差分析确定各组间的差异。3%氯化钠可减轻脂多糖引起的脑组织含水量、IL-1β、肿瘤坏死因子α、Ig G、AQP4m RNA和蛋白含量的增加。3%氯化钠可抑制IL-1β诱导的星形胶质细胞AQP4mRNA和蛋白表达的增加。Calphostin C可阻断3%氯化钠诱导的星形胶质细胞AQP4基因和蛋白表达的下降。3%氯化钠渗透疗法可减轻在体脂多糖诱导的脑水肿。3%氯化钠除具有渗透力外，还可能通过下调促炎细胞因子(IL-1β和肿瘤坏死因子α)的表达和抑制促炎细胞因子诱导的水通道蛋白4的表达而发挥抗水肿作用。3%的氯化钠通过激活蛋白激酶C来抑制AQP4的表达。
Three percent sodium chloride (NaCl) treatment has been shown to reduce brain edema and inhibited brain aquaporin 4 (AQP4) expression in bacterial meningitis induced by Escherichia coli. Lipopolysaccharide (LPS) is the main pathogenic component of E. coli. We aimed to explore the effect of 3% NaCl in mouse brain edema induced by LPS, as well as to elucidate the potential mechanisms of action. Three percent NaCl was used to treat cerebral edema induced by LPS in mice in vivo. Brain water content, IL-1β, TNFα, immunoglobulin G (IgG), AQP4 mRNA and protein were measured in brain tissues. IL-1β, 3% NaCl and calphostin C (a specific inhibitor of protein kinase C) were used to treat the primary astrocytes in vitro. AQP4 mRNA and protein were measured in astrocytes. Differences in various groups were determined by one-way analysis of variance. Three percent NaCl attenuated the increase of brain water content, IL-1β, TNFα, IgG, AQP4 mRNA and protein in brain tissues induced by LPS. Three percent NaCl inhibited the increase of AQP4 mRNA and protein in astrocytes induced by IL-1β in vitro. Calphostin C blocked the decrease of AQP4 mRNA and protein in astrocytes induced by 3% NaCl in vitro. Osmotherapy with 3% NaCl ameliorated LPS-induced cerebral edema in vivo. In addition to its osmotic force, 3% NaCl exerted anti-edema effects possibly through down-regulating the expression of proinflammatory cytokines (IL-1β and TNFα) and inhibiting the expression of AQP4 induced by proinflammatory cytokines. Three percent NaCl attenuated the expression of AQP4 through activation of protein kinase C in astrocytes.
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影响因子：
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