Human macrophage adhesion on fibronectin: the role of substratum and intracellular signalling kinases.
Human macrophage adhesion on fibronectin: the role of substratum and intracellular signalling kinases.
复制标题
人巨噬细胞对纤连蛋白的粘附：基质和细胞内信号激酶的作用。
DOI：
10.1016/s0898-6568(01)00246-7
复制
发表时间：
2002
影响因子：
4.8
通讯作者：
Kao,WeiyuanJohn
中科院分区：
文献类型：
--
作者：
Liu,Yiping;Kao,WeiyuanJohn
Fibronectin and Arg-Gly-Asp (RGD)- and/or Pro-His-Ser-Arg-Asn (PHSRN)-containing oligopeptides were immobilized onto physicochemically distinct substrata: polyethyleneglycol-based networks or tissue culture polystyrene (TCPS). The role of selected signalling kinases in the adhesion of human primary blood-derived macrophages on these modified substrata was investigated. We demonstrated that the protein tyrosine kinase (PTK) or protein serine/threonine kinase (PSK) dependency and the PTK–PSK cross-talk compensation for macrophage adhesion varied dynamically with the substratum modification and the culture time. The inhibition of MAPK kinase (MAPKK) decreased macrophage adhesion on TCPS, whereas the inhibition of phosphoinositide-3 kinase (PI3 kinase) decreased macrophage adhesion on networks at 24 h. The PI3 kinase–protein kinase C (PKC)–MAPK cascade was involved in macrophage adhesion on fibronectin-preadsorbed TCPS or networks but not on fibronectin-grafted networks. This fibronectin-mediated adhesion signalling involved both RGD and PHSRN sequences in a form of G3RGDG6PHSRNG on TCPS but not on networks. Furthermore, G3RGDG6PHSRNG grafted onto networks evoked unique signalling in macrophage adhesion from that preadsorbed onto networks. Thus, macrophage adhesion and the role of selected signalling kinases were modulated by the substratum and the ligand conjugation method.
