Physiological identification and analysis of dentate granule cell responses to stimulation of the medial and lateral perforant pathways in the rat
Physiological identification and analysis of dentate granule cell responses to stimulation of the medial and lateral perforant pathways in the rat
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大鼠齿状颗粒细胞对内侧和外侧穿通通路刺激反应的生理鉴定和分析
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
C. Barnes
中科院分区：
文献类型：
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作者：
B. McNaughton;C. Barnes
刺激背内侧或腹外侧穿支通路导致定量不同的细胞外记录的EPSP在大鼠的齿筋膜。这两个潜在的不同的潜伏期峰值和宽度在半振幅的方式一致的不同位点的终止的两个途径上的颗粒细胞树突。这两种电位都能够跟随100 Hz的短暂刺激串，这表明它们是单突触的。中间引发的反应在分子层中的峰值负性比横向引发的反应深约100至180 μm。沿角束的背内侧到腹外侧轨迹以短间隔沿着刺激产生阶跃函数，而不是EPSP峰潜伏期和半宽的连续体，与Hjorth‐Simonsen（'72）关于两条通路分离的证据一致。这两种途径都能诱导颗粒细胞放电。然而，横向引起的尖峰被延迟。在中间位置的刺激经常引起颗粒细胞群的双尖峰。由任一途径引起的群体尖峰在单次放电后被抑制长达100毫秒。两条通路均显示短时间间隔双重刺激的易化作用，并且均显示持续至少30分钟的强直后增强作用。在可以表明两条通路至少部分会聚到相同颗粒细胞的条件下，当在另一条通路上诱导长持续增强时，一条通路的反应并不增加。
Stimulation of the dorsomedial or ventrolateral perforant pathways resulted in quantitatively different extracellularly recorded EPSPs in the fascia dentata of the rat. The two potential differed in latency to peak and in width at half amplitude in a manner consistent with the different locus of termination of the two pathways on the granule cell dendrites. Both potentials were able to follow brief stimulus trains of 100 Hz, which suggests that they are monosynaptic. Medially elicited responses had their peak negativity approximately 100 to 180 μm deeper in the molecular layer than laterally elicited responses. Stimulation at short intervals along a dorsomedial to ventrolateral track in the angular bundle yielded a step function rather than a continuum of EPSP peak latency and half‐width, in agreement with Hjorth‐Simonsen's ('72) evidence for the separateness of the two pathways. Both pathways were able to induce granule cell discharge. Laterally elicited spikes, however, were delayed. Stimulation at intermediate locations frequently elicited double spikes from granule cell population. Population spikes elicited by either pathway were inhibited for as long as 100 msec after a single discharge. Both pathways showed facilitation with double stimuli at short intervals, and both showed post‐tetanic potentiation lasting at least 30 minutes. Under conditins where it could be shown that the two pathways at least partially converged onto the same granule cells, the response of one pathway did not increase when long lasting potentiation was induced on the other.