Stress increases gene expression of phenylethanolamine N-methyltransferase in spleen of rats via pituitary-adrenocortical mechanism.
Stress increases gene expression of phenylethanolamine N-methyltransferase in spleen of rats via pituitary-adrenocortical mechanism.
复制标题
压力通过垂体-肾上腺皮质机制增加大鼠脾脏中苯乙醇胺 N-甲基转移酶的基因表达。
DOI：
10.1016/s0306-4530(01)00098-1
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发表时间：
2002
影响因子：
3.7
通讯作者：
Kvetnansky,R
中科院分区：
文献类型：
--
作者：
Jelokova,J;Rusnak,M;Kubovcakova,L;Buckendahl,P;Krizanova,O;Sabban,EL;Kvetnansky,R
使用逆转录聚合酶链式反应在大鼠脾脏中检测到苯乙醇胺 N-甲基转移酶 (PNMT) 的基因表达，PNMT 是一种催化去甲肾上腺素转化为肾上腺素的酶。随后通过 Southern 印迹验证 PNMT 身份。通过原位杂交研究了负责 PNMT 基因表达的脾细胞的定位，发现 PNMT mRNA 存在于白髓中。测试了应激可能导致大鼠脾脏中 PNMT 基因表达增加的假设，并在单次或重复固定后观察到 PNMT mRNA 水平相对丰度的强劲上升（约 80%）。肾上腺切除术或垂体切除术完全阻止了固定诱导的脾脏 PNMT mRNA 水平的增加，表明应激诱导的脾脏 PNMT 基因表达主要通过垂体-肾上腺皮质轴调节。然而，在对照动物中，脾脏 PNMT 并未受到切除术的显着影响，因此基础 PNMT 基因表达可能受到不同机制的调节。因此，应激刺激会增强大鼠脾脏中的 PNMT 基因表达，表明其在生理调节中的作用。
Gene expression of phenylethanolamine N-methyltransferase (PNMT), the enzyme catalyzing conversion of norepinephrine to epinephrine, has been detected in rat spleen using the reverse transcription polymerase chain reaction. PNMT identity was subsequently verified by Southern blots. Localization of the spleen cells responsible for the PNMT gene expression was investigated by the in situ hybridization and PNMT mRNA was found to be present in the white pulp. The hypothesis that stress may produce an increase in PNMT gene expression in rat spleen was tested and a robust rise in the relative abundance of PNMT mRNA levels was observed after a single or repeated immobilization (about 80%). Adrenalectomy or hypophysectomy completely prevented the immobilization-induced increase in spleen PNMT mRNA levels, suggesting that stress-induced PNMT gene expression in the spleen is regulated predominantly via pituitary-adrenocortical axis. In control animals, however, spleen PNMT was not significantly affected by the ectomies and therefore basal PNMT gene expression might be regulated by different mechanism(s). Thus, PNMT gene expression in the rat spleen is exaggerated by stress stimuli, suggesting its role in physiological regulations.