Retrograde control of synaptic transmission by postsynaptic CaMKII at Drosophila neuromuscular
Retrograde control of synaptic transmission by postsynaptic CaMKII at Drosophila neuromuscular
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DOI：
10.1016/s0896-6273(03)00427-6
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发表时间：
2003-07-17
期刊：
影响因子：
16.2
通讯作者：
Goodman, CS
中科院分区：
文献类型：
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作者：
Haghighi, AP;McCabe, BD;Goodman, CS
逆行信号传导在突触稳态、生长和可塑性中起重要作用。果蝇神经肌肉接头（NMJ）的逆行信号控制着神经递质释放的稳态。在这里，我们表明，这种逆行信号是由突触后活性的Ca 2 +/钙调蛋白依赖性蛋白激酶II（CaMKII）。降低肌肉中的CaMKII活性增强信号并增加神经递质释放，而肌肉中CaMKII的组成性激活抑制信号并减少神经递质释放。突触后抑制CaMKII增加突触前，囊泡相关的T酒吧在活动区的数量。一致的，我们表明，谷氨酸受体突变体也有更多的T酒吧，这种增加被抑制突触后激活的CaMKII。此外，我们证明，突触前BMP受体一厢情愿的逆行信号的功能。我们的研究结果表明，CaMK Ⅱ在果蝇NMJ突触传递的稳态逆行控制中起着关键作用。
Retrograde signaling plays an important role in synaptic homeostasis, growth, and plasticity. A retrograde signal at the neuromuscular junction (NMJ) of Drosophila controls the homeostasis of neurotransmitter release. Here, we show that this retrograde signal is regulated by the postsynaptic activity of Ca2+/calmodulin-dependent protein kinase II (CaMKII). Reducing CaMKII activity in muscles enhances the signal and increases neurotransmitter release, while constitutive activation of CaMKII in muscles inhibits the signal and decreases neurotransmitter release. Postsynaptic inhibition of CaMKII increases the number of presynaptic, vesicle-associated T bars at the active zones. Consistently, we show that glutamate receptor mutants also have a higher number of T bars; this increase is suppressed by postsynaptic activation of CaMKII. Furthermore, we demonstrate that presynaptic BMP receptor wishful thinking is required for the retrograde signal to function. Our results indicate that CaMKII plays a key role in the retrograde control of homeostasis of synaptic transmission at the NMJ of Drosophila.