Emergence of group a streptococcus strains with different mechanisms of macrolide resistance
Emergence of group a streptococcus strains with different mechanisms of macrolide resistance
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DOI：
10.1128/aac.46.5.1199-1203.2002
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发表时间：
2002-05-01
影响因子：
4.9
通讯作者：
Cohen, R
中科院分区：
文献类型：
--
作者：
Bingen, E;Leclercq, R;Cohen, R
7株A组链球菌（化脓性链球菌）对大环内酯类药物的耐药机制进行了评估，这些菌株是阿奇霉素（10 mg/kg体重/天，连续3天）治疗失败的儿童咽炎的原因。通过随机扩增多态性DNA分析和脉冲场凝胶电泳分析，发现所有后处理菌株与前处理菌株具有遗传相关性。两个分离株获得了mef(a)或erm(B)基因，分别负责大环内酯外排和核糖体修饰。三个分离株显示编码L4核糖体蛋白的基因突变，L4核糖体蛋白是细菌核糖体50S亚基出口通道的一部分。在其余两株处理后菌株中，大环内酯耐药机制尚不清楚。
The mechanisms of resistance to macrolides in seven group A streptococcal (Streptococcuspyogenes) isolates that were the cause of pharyngitis in children who were unsuccessfully treated with azithromycin (10 mg/kg of body weight/day for 3 days) were evaluated. All posttreatment strains were found to be genetically related to the pretreatment isolates by random amplified polymorphism DNA analysis and pulsed-field gel electrophoresis. Two isolates had acquired either a mef(A) or an erm(B) gene, responsible for macrolide efflux and ribosomal modification, respectively. Three isolates displayed mutations in the gene encoding the L4 ribosomal protein that is part of the exit tunnel within the 50S subunit of the bacterial ribosome. In the two remaining posttreatment strains, the mechanisms of macrolide resistance could not be elucidated.