Association between kallikrein-related peptidases (KLKs) and macroscopic indicators of semen analysis: their relation to sperm motility
Association between kallikrein-related peptidases (KLKs) and macroscopic indicators of semen analysis: their relation to sperm motility
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DOI：
10.1515/bc.2009.094
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发表时间：
2009-09-01
影响因子：
3.7
通讯作者：
Diamandis, Eleftherios P.
中科院分区：
文献类型：
--
作者：
Emami, Nashmil;Scorilas, Andreas;Diamandis, Eleftherios P.
人激肽释放酶相关多肽酶(KLK)是一类蛋白水解酶家族，主要存在于精浆中，与精液液化有关。在这里，我们研究了精液KLK在精液质量评估、液化和/或粘度异常的鉴别诊断和病因学方面的临床价值。KLK1-3、5-8、10、11、13和14用高度特异的酶联免疫吸附试验进行分析。根据液化状态和粘度将样本分为四个临床组。临床组之间的数据进行了比较，并与其他精子质量参数进行了关联，包括活动精子数、直线速度、精子密度、体积、pH值和患者年龄。精液KLK在四组临床病例中均有不同程度的表达。KLK2、3、13和14与KLK1、2、5、6、7、8、10、13和14的组合分别对精液液化和粘度具有很强的区分能力。液化状态与精子活力的几个参数有关。最后，KLK14在弱精子症患者中有差异表达。总之，几种精浆KLK的表达水平与精液质量的液化和粘度指标相关，并可能有助于它们的鉴别诊断和病因。
Human kallikrein-related peptidases (KLKs) are a family of proteases, the majority of which are found in seminal plasma and have been implicated in semen liquefaction. Here, we examined the clinical value of seminal KLKs in the evaluation of semen quality and differential diagnosis and etiology of abnormal liquefaction and/or viscosity. KLK1-3, 5-8, 10, 11, 13, and 14 were analyzed, using highly specific ELISA assays. Samples were categorized into four clinical groups, according to their state of liquefaction and viscosity. Data were compared between the clinical groups and in association with other parameters of sperm quality, including number of motile sperms, straight line speed, sperm concentration, volume, pH, and patient age. Seminal KLKs were found to be differentially expressed in the four clinical groups. Combination of KLK2, 3, 13, and 14 and KLK1, 2, 5, 6, 7, 8, 10, 13, and 14 showed very strong discriminatory potential for semen liquefaction and viscosity, respectively. Liquefaction state was associated with several parameters of sperm motility. Finally, KLK14 was differentially expressed in asthenospermic cases. In conclusion, the expression level of several seminal plasma KLKs correlates with liquefaction and viscosity indicators of semen quality and may aid in their differential diagnosis and etiology.