Identification and mapping of the promoter for the gene encoding the ferritin heavy-chain homologue of the yellow fever mosquito Aedes aegypti.
Identification and mapping of the promoter for the gene encoding the ferritin heavy-chain homologue of the yellow fever mosquito Aedes aegypti.
复制标题
编码黄热病蚊子埃及伊蚊铁蛋白重链同源物的基因启动子的鉴定和定位。
DOI：
10.1016/s0965-1748(02)00167-4
复制
发表时间：
2003
影响因子：
3.8
通讯作者：
Douglass,PL
中科院分区：
文献类型：
--
作者：
Pham,DQ-D;Shaffer,JJ;Chavez,CA;Douglass,PL
蚊子是传播许多人类疾病的罪魁祸首。转基因蚊子的最新发展为研究昆虫媒介及其传播的病原体之间的分子相互作用提供了新的工具。转基因蚊子的一个重点是在致病性入侵的主要部位，特别是蚊子肠道中表达抗致病性蛋白。因此，在蚊子肠道中指导抗致病性蛋白表达的启动子受到追捧，因为它们可能提供阻碍蚊子致病性发展的途径。在这里，我们报道了埃及伊蚊铁蛋白重链同源基因（HCH）的一个强启动子的鉴定和定位。所有已知的昆虫铁蛋白HCH基因都在肠道中表达，并由铁过载诱导。我们的转染实验和dna酶I足迹分析表明，蚊子铁蛋白hch基因在转录起始位点的上游和下游都含有调控元件。该基因的启动子包含一个CF2位点、两个gata结合位点、一个E2F位点、一个TATA-box位点、一个AP-1位点和一个C/EBP结合位点。
Mosquitoes are responsible for the transmission of numerous human diseases. The recent development of transgenic mosquitoes provides a new tool to examine molecular interactions between insect vectors and the pathogens they transmit. One focus in generating transgenic mosquito lies on expressing anti-pathogenic proteins at primary sites of pathogenic invasions, specifically the mosquito gut. Promoters that direct the expression of anti-pathogenic proteins in the mosquito gut are thus sought after because they may provide ways to hinder pathogenic development in the mosquito. Here, we report the identification and mapping of a strong promoter from the Aedes aegypti ferritin heavy-chain homologue (HCH) gene. All known insect ferritin HCH genes are expressed in the gut and inducible by an iron overload. Our transfection assays and DNase I footprinting analyses show that the mosquito ferritin HCH-gene contains regulatory elements both upstream and downstream of the transcriptional start site. The promoter of this gene contains a CF2 site, two GATA-binding sites, an E2F site, a TATA-box, an AP-1 site and a C/EBP binding site.