Presence of 1-hydroxypyrene conjugates in woody plant leaves and seasonal changes in their concentrations
Presence of 1-hydroxypyrene conjugates in woody plant leaves and seasonal changes in their concentrations
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DOI：
10.1021/es950665e
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发表时间：
1996-05-01
影响因子：
11.4
通讯作者：
Suzuki, S
中科院分区：
文献类型：
--
作者：
Nakajima, D;Kojima, E;Suzuki, S
在四种木本植物：樱桃、枫树、银杏和樟脑的叶子中发现了1-羟基芘（OHPy）的β-O-葡萄糖苷和β-O-葡萄糖苷酸结合物。通过测量从用β-O-葡糖苷酶和β-O-葡糖醛酸糖苷酶处理的叶的水溶性级分释放的OHPy来定量缀合物。β-O-葡糖苷酸和β-O-葡萄糖苷的浓度范围分别为0至358和0至129 pmol/g鲜叶，每月测量一年。在大多数植物中，葡萄糖苷酸结合物的量大于葡萄糖苷结合物的量。两种结合物均在夏季至秋季以最高浓度检出。对于大多数植物，结合物的总量大于游离的OHPy在所有季节的叶片中，9月的枫叶的差异达到30倍。从这些结果，在叶中的OHPy共轭物的来源和它们的影响进行了讨论。
beta-O-Glucoside and beta-O-glucuronide conjugates of 1-hydroxypyrene (OHPy) were found in the leaves of four kinds of woody plant; cherry, maple, ginkgo, and camphor. The conjugates were quantified by measuring OHPy liberated from the water-soluble fraction of leaves treated with beta-O-glucosidase and beta-O-glucuronidase. Concentrations of beta-O-glucuronide and beta-O-glucoside ranged from 0 to 358 and from 0 to 129 pmol/g of fresh leaves, respectively, in monthly measurement over a period of 1 year. The amounts of glucuronide conjugates were greater than those of glucoside conjugates in most plants examined. Both conjugates were detected at the highest concentrations in summer to autumn. For most plants, the total amounts of the conjugates were greater than that of free OHPy in the leaves in all seasons, and the differences reached 30-fold in maple leaves in September. From these results, the source of the OHPy conjugates in leaves and their implications are discussed.