Application of molecular spectroscopy in the mid-infrared region to the determination of glucose and cholesterol in whole blood and in blood serum
Application of molecular spectroscopy in the mid-infrared region to the determination of glucose and cholesterol in whole blood and in blood serum
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DOI：
10.1366/0003702971941034
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发表时间：
1997-05-01
影响因子：
3.5
通讯作者：
Volka, K
中科院分区：
文献类型：
--
作者：
Budinova, G;Salva, J;Volka, K
通过在聚乙烯载体上测量干燥状态下的样品，验证了分子光谱在中红外光谱应用于全血和血清中葡萄糖和胆固醇定量的可行性，采用偏最小二乘算法建立了校正模型。通过将光谱归一化到标准区域进行分析，通过以硫氰酸钾为内标的实验获得了这种方法可靠的证据。校准后得到的平均PRESS1/2值(PRESS1/2代表预测残差平方和)：葡萄糖22.3 mg/dL；胆固醇31.3 mg/dL。这种误差与衰减全反射(ATR)测量的误差相当，建立的校准模型远比全血样本拟合得更好。如果改进载体上的进样程序，该方法可用于全血和血清中葡萄糖和胆固醇的测定。
The feasibility of applying molecular spectroscopy in the mid-infrared region to the quantitation of glucose and cholesterol in whole blood and in blood serum was examined by measuring the samples in the dry state on a polyethylene carrier, The partial least-squares (PLS) algorithm was employed to set up the calibration models. Analysis was performed by normalizing the spectra to a standard area, Evidence that this approach is warranted was obtained through experiments where potassium thiocyanate served as the internal standard. The following average PRESS1/2 values (PRESS stands for prediction residual error sum of squares) emerged from the calibration: glucose, 22.3 mg/dL; cholesterol, 31.3 mg/dL. Such errors are comparable with those achieved in attenuated total reflectance (ATR) measurements, The calibration models set up far blood serum samples fit better than those for whole blood samples. If the procedure of sample application onto the carrier is improved, the method may work well for the determination of glucose and cholesterol in whole blood and in blood serum.