Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions
Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions
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DOI：
10.1111/j.1567-1364.2002.tb00117.x
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发表时间：
2002-12-01
影响因子：
3.2
通讯作者：
Statzell-Tallman, A
中科院分区：
文献类型：
--
作者：
Scorzetti, G;Fell, JW;Statzell-Tallman, A
对解脲支原体和膜菌属担子菌的大亚基rRNA基因5‘端的D_1/D_2可变区(D_1/D_2)和内部转录间隔区(ITS)1和2的核糖体DNA区域进行了序列分析。每类均可识别出四个主要谱系：解脲支原体中的微孢子菌属、孢子球菌属、红曲霉属和琼脂糖酵母菌；对这些谱系中的许多支系的自举支持很弱；然而，系统学分析为深入研究生物关系提供了一个焦点。对于物种的鉴定，推荐使用结合序列分析的方法，包括对D1/D2区和ITS区的分析，而物种的定义则需要经典的生物学信息，如生命周期和表型特征。(C)2002年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
Basidiomycetous yeasts in the Urediniomycetes and Hymenomycetes were examined by sequence analysis in two ribosomal DNA regions: the D1/D2 variable domains at the 5' end of the large subunit rRNA gene (D1/D2) and the internal transcribed spacers (ITS) 1 and 2. Four major lineages were recognized in each class: Microbotryum, Sporidiobolus, Erythrobasidium and Agaricostilbum in the Urediniomycetes; Tremellales, Trichosporonales, Filobasidiales and Cystofilobasidiales in the Hymenomycetes. Bootstrap support for many of the clades within those lineages is weak; however, phylogenctic analysis provides a focal point for in-depth study of biological relationships. Combined sequence analysis of the D1/D2 and ITS regions is recommended for species identification, while species definition requires classical biological information such as life cycles and phenotypic characterization. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.