Purification and stabilization of mouse DNA methyltransferase.
Purification and stabilization of mouse DNA methyltransferase.
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小鼠 DNA 甲基转移酶的纯化和稳定。
DOI：
10.1006/bbrc.1995.1222
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发表时间：
1995
影响因子：
3.1
通讯作者：
Reich,NO
中科院分区：
文献类型：
--
作者：
Xu,G;Flynn,J;Glickman,JF;Reich,NO
DNA内的胞嘧啶甲基化与遗传印记、x染色体失活、组织特异性基因表达调控、衰老和癌症有关。不幸的是，来自各种哺乳动物来源的DNA（胞嘧啶-5）-甲基转移酶（EC 2.1.1.37）难以分离和稳定，阻碍了这些关键酶的研究。我们描述了一种从小鼠Friend红白血病细胞中纯化190,000 Mr DNA甲基转移酶的新型FPLC。该酶的均匀190 kD Mr形式是唯一在细胞生长的各个阶段检测到的多肽，并且没有经过可检测到的n端蛋白水解。
Cytosine methylation within DNA has been implicated in genetic imprinting, X-chromosome inactivation, regulation of tissue-specific gene expression, aging, and cancer. Unfortunately, DNA (cytosine-5)-methyltransfersases (EC 2.1.1.37) from various mammalian sources have been difficult to isolate and stabilize, precluding investigations of these critical enzymes. We describe a novel FPLC purification of the 190,000 Mr DNA methyltransferase from mouse Friend erythroleukemia cells. The homogeneous 190 kD Mr form of the enzyme is the only polypeptide detected at various stages of cell growth and has not undergone detectable N-terminal proteolysis.