Expression of avian β-defensins in the oviduct and effects of lipopolysaccharide on their expression in the vagina of hens
Expression of avian β-defensins in the oviduct and effects of lipopolysaccharide on their expression in the vagina of hens
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DOI：
10.3382/ps.2007-00283
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发表时间：
2008-05-01
期刊：
影响因子：
4.4
通讯作者：
Yoshimura, Y.
中科院分区：
文献类型：
--
作者：
Mageed, A. M. Abdel;Isobe, N.;Yoshimura, Y.
本研究的目的是(1)确定鸡输卵管中表达的禽β-防御素基因(AvβD)的类型；(2)研究体内脂多糖(LPS)处理对其在阴道中表达的影响。静脉注射脂多糖(1 mg/kg体重)，分别于注射后0、3、6、12、24 h取输卵管进行分析。用逆转录-聚合酶链式反应检测所有输卵管段粘膜组织中AvβD的mRNA表达。用半定量逆转录-聚合酶链式反应(RT-PCR)分析其在阴道中的表达变化。AvβD-1、-2、-3、-4、-5、-7、-8、-9、-10、-11和-12在输卵管漏斗至阴道各段均有表达。其中，内毒素刺激后，阴道组织中A vβD-3、-5、-10、-11、-12的表达显著增加，其余均无明显变化。这些结果表明，11种类型的AvβD在母鸡输卵管中均有表达，其中至少有5种在阴道中的表达对内毒素有反应。
The aims of this study were to (i) determine the types of avian beta-defensin genes (Av beta D) expressed in the hen oviduct and (ii) to examine the effects of lipopolysaccharide (LPS) treatment in vivo on their expression in the vagina. Birds were i. v. treated with LPS (1 mg/kg of BW), and subsequently the oviducts were analyzed 0, 3, 6, 12, or 24 h after LPS administration. The mRNA expression for Av beta D was examined by reverse transcription-PCR using RNA preparations from the mucosal tissues of all the oviductal segments. Furthermore, changes in their mRNA expression profiles in the vagina were analyzed by semiquantitative reverse transcription-PCR. The Av beta D-1, -2, -3, -4, -5, -7, -8, -9, -10, -11, and -12 were identified in each oviductal segment from infundibulum to vagina. Among these Av beta D, the expression of Av beta D-3, -5, -10, -11, and -12 in the vagina were significantly increased in response to LPS treatment, whereas the others did not show significant changes. These results suggest that all 11 types of Av beta D are expressed in the hen oviduct and at least 5 of them in the vagina show increased expression in response to LPS.