A novel approach for scanning electron microscopy of colloidal gold-labeled cell surfaces.
A novel approach for scanning electron microscopy of colloidal gold-labeled cell surfaces.
复制标题
DOI：
10.1083/jcb.99.1.53
复制
发表时间：
1984-07
期刊：
影响因子：
--
通讯作者：
Christensen H
中科院分区：
文献类型：
--
作者：
de Harven E;Leung R;Christensen H
描述了一种在金标记细胞表面使用扫描电子显微镜的方法。它包括使用与葡萄球菌蛋白 A 结合的 45 或 20 nm 胶体金标记。通过使用扫描电子显微镜的背散射电子成像模式，根据其原子序数对比可以最好地识别该标记。当背散射电子信号与二次电子信号混合时，标记位点的分布和细胞表面结构之间的最佳相关性被证明。该方法通过其在人类循环粒细胞鉴定中的应用来说明。其良好的分辨率、高对比度和良好的标记效率为胶体金颗粒标记的细胞表面抗原位点的特异性定位提供了一种有前途的方法。
A method is described for the use of scanning electron microscopy on the surface of gold-labeled cells. It includes the use of 45- or 20-nm colloidal gold marker conjugated with Staphylococcal protein A. The marker is best recognized on the basis of its atomic number contrast by using the backscattered electron imaging mode of the scanning electron microscope. When the backscattered electron signal is mixed with the secondary electron signal, an optimum correlation between the distribution of the labeled sites and the cell surface structures is demonstrated. The method is illustrated by its application to the identification of human circulating granulocytes. Its good resolution, high contrast, and good labeling efficiency offers a promising approach to the specific localization of cell surface antigenic sites labeled with particles of colloidal gold.