The Mauthner cell in a fish with top-performance and yet flexibly tuned C-starts. II. Physiology
The Mauthner cell in a fish with top-performance and yet flexibly tuned C-starts. II. Physiology
复制标题
DOI：
10.1242/jeb.175588
复制
发表时间：
2018-07-01
影响因子：
2.8
通讯作者：
Schuster, Stefan
中科院分区：
文献类型：
--
作者：
Machnik, Peter;Leupolz, Kathrin;Schuster, Stefan
射水鱼身上同时出现了经过微调但功能强大的预测性 C-starts 以及运动学上相同的逃逸 C-starts，这使得射水鱼成为一个有趣的系统，可以用来测试关于 Mauthner 细胞（一对巨大的网状脊髓神经元）所扮演的角色的假设。在这项研究中，我们证明射水鱼毛特纳细胞与金鱼具有所有标志性的生理特性。视觉和听觉输入分别由腹侧和外侧树突接收，即使在手术麻醉的鱼中也会引起复杂的突触后电位（PSP）。 PSP 形状并不表明物种之间存在重大差异，但简单的闪光会导致射水鱼的 PSP 变大，通常会驱动细胞激发动作电位。在金鱼莫特纳相关网络中建立的反馈抑制经典测试中探测射水鱼，发现这两个物种之间没有差异，包括电和化学突触成分的指示。此外，已建立的前馈抑制标志性实验表明金鱼和射水鱼毛特纳系统之间没有差异。将这些实验扩展到视觉刺激也未能检测到两个物种之间的任何差异，并表明声学和视觉输入会导致前馈抑制，其幅度、时间过程和持续时间与射水鱼和金鱼各自的 PSP 相匹配。我们的研究结果质疑了关于 Mauthner 细胞作用的简单观点，并表明射水鱼 Mauthner 细胞应该是一个很好的系统，可以探索这些巨型神经元在更复杂的 C-start 行为中的功能。
The parallel occurrence in archerfish of fine-tuned and yet powerful predictive C-starts as well as of kinematically identical escape C-starts makes archerfish an interesting system to test hypotheses on the roles played by the Mauthner cells, a pair of giant reticulospinal neurons. In this study, we show that the archerfish Mauthner cell shares all hallmark physiological properties with that of goldfish. Visual and acoustic inputs are received by the ventral and lateral dendrite, respectively, and cause complex postsynaptic potentials (PSPs) even in surgically anaesthetised fish. PSP shape did not indicate major differences between the species, but simple light flashes caused larger PSPs in archerfish, often driving the cell to fire an action potential. Probing archerfish in the classical tests for feedback inhibition, established in the Mauthner-associated networks in goldfish, revealed no differences between the two species, including the indications for electrical and chemical synaptic components. Also, the established hallmark experiments on feed-forward inhibition showed no differences between the goldfish and archerfish Mauthner system. Extending these experiments to visual stimuli also failed to detect any differences between the two species and suggested that acoustical and visual input cause feed-forward inhibition, the magnitude, time course and duration of which match that of the respective PSPs in both archerfish and goldfish. Our findings question simple views on the role of the Mauthner cell and suggest that the archerfish Mauthner cell should be a good system to explore the function of these giant neurons in more sophisticated C-start behaviours.