Luteolin Regulation of Estrogen Signaling and Cell Cycle Pathway Genes in MCF-7 Human Breast Cancer Cells
Luteolin Regulation of Estrogen Signaling and Cell Cycle Pathway Genes in MCF-7 Human Breast Cancer Cells
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DOI：
10.59566/ijbs.2011.7101
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发表时间：
2011-06
期刊：
影响因子：
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通讯作者：
B. Markaverich;K. Shoulars;M. Rodriguez
中科院分区：
文献类型：
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作者：
B. Markaverich;K. Shoulars;M. Rodriguez
cRNA微阵列和实时PCR （qPCR）研究在MCF-7乳腺癌细胞中发现了一些雌激素信号通路（ESP）基因（GTF2H2、NCOR1、TAF9、NRAS、NRIP1、POLR2A、DDX5、NCOA3）和细胞周期通路基因（CCNA2、PCNA、CDKN1A、CCND1、PLK1）受生物类黄酮木草素调控。染色质免疫沉淀（ChIP）研究表明木犀草素修饰了PLK-1启动子上组蛋白H4乙酰化，表明这种生物类黄酮通过一种涉及组蛋白H4乙酰化的表观遗传机制来控制基因转录。这些发现与木犀草素在正常和恶性细胞中的抗雌激素和抗增殖特性是一致的。
cRNA microarray and real-time PCR (qPCR) studies identified a number of Estrogen Signaling Pathway (ESP) genes (GTF2H2, NCOR1, TAF9, NRAS, NRIP1, POLR2A, DDX5, NCOA3) and Cell Cycle Pathway genes (CCNA2, PCNA, CDKN1A, CCND1, PLK1) in MCF-7 breast cancer cells that are regulated by the bioflavonoid luteolin. Chromatin immunoprecipitation (ChIP) studies revealed that luteolin modified histone H4 acetylation at the PLK-1 promoter suggesting that this bioflavonoid controls gene transcription via an epigenetic mechanism involving histone H4 acetylation. These findings are consistent with the anti-estrogenic and anti-proliferative properties of luteolin in normal and malignant cells.