Quantification of the Influence of Endotoxins on the Mechanics of Adult and Neonatal Red Blood Cells
Quantification of the Influence of Endotoxins on the Mechanics of Adult and Neonatal Red Blood Cells
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内毒素对成人和新生儿红细胞力学影响的定量
DOI：
10.1021/acs.jpcb.5b01544
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
and Motomu Tanaka
中科院分区：
文献类型：
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作者：
Hiroaki Ito;Navina Kuss;Bastian E. Rapp;Masatoshi Ichikawa;Thomas Gutsmann;Klaus Brandenburg;Johannes M. B. Poeschl;and Motomu Tanaka
在这项研究中，我们通过傅立叶变换的形状波动均方幅度分析(称为闪烁光谱分析)来量化内毒素对细胞力学的影响，从而物理模拟内毒素诱导的脓毒症症状对人红细胞(RBC)的影响。借助于微流控扩散室，我们首次无创性地测定了单个红细胞在无内毒素和有内毒素存在的情况下的主要力学参数。由于内毒素的糖链长度延长，我们发现从盘状细胞到棘细胞的形态转变增加，力学参数发生单调变化。由于感染性休克常常导致新生儿死亡的危险，我们测量了新生儿红细胞的力学参数，并与成人红细胞的力学参数进行了比较。定量比较表明，新生儿红细胞比成人红细胞更容易受到内毒素的影响。此外，与抗菌肽P19-2.5(Aspidasept)与内毒素共同孵育可轻微抑制内毒素的影响。本研究提出的策略有可能应用于基于机械读数的红细胞定量诊断。
In this study, we physically modeled the influence of endotoxin-induced sepsis symptoms on human red blood cells (RBCs) by quantifying the impact of endotoxins on the cell mechanics by the analysis of Fourier-transformed mean square amplitude of shape fluctuation, called flicker spectroscopy. With the aid of a microfluidic diffusion chamber, we noninvasively determined principal mechanical parameters of human RBCs in the absence and presence of endotoxins for individual RBCs for the first time. Because of the elongation of saccharide chain length of endotoxins, we found an increase in the morphological transition from discocytes to echinocytes, and monotonic changes in the mechanical parameters. Since septic shocks often cause lethal risks of neonates, we measured the mechanical parameters of neonatal RBCs, and compared them to those of adult RBCs. The quantitative comparison reveals that neonatal RBCs are more susceptible to the effect of endotoxins than adult RBCs. Furthermore, coincubation with the antiseptic peptide P19-2.5 (Aspidasept) with endotoxin results in a slight suppression of the impact of the endotoxin. The strategy proposed in our study can potentially be applied for the quantitative diagnosis of RBCs based on mechanical readouts.