Glycosaminoglycan hydrogel films as bio-interactive dressings for wound healing
Glycosaminoglycan hydrogel films as bio-interactive dressings for wound healing
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DOI：
10.1016/s0142-9612(02)00100-x
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发表时间：
2002-09-01
期刊：
影响因子：
14
通讯作者：
Prestwich, GD
中科院分区：
文献类型：
--
作者：
Kirker, KR;Luo, Y;Prestwich, GD
制备了化学交联型糖胺聚糖(GAG)水凝胶膜，并对其作为生物交互创面敷料进行了评价。首先将透明质酸(HA)和硫酸软骨素(CS)转化为己二酸二肼类化合物，然后与聚乙二醇丙二醛交联制得聚合物网络。在室温下，在中性pH条件下，几分钟内发生交联，得到透明、柔软的水凝胶。凝胶化后，采用溶剂浇铸法制备GAG水凝胶膜。小鼠模型被用来评估这些GAG膜在促进伤口愈合方面的效果。在Balb/c小鼠背侧造成全层创面，并用GAG膜加Tegaderm(TM)或Tegaderm(TM)单独包扎。在第5天(p<；0.001)和第7天(p<；005)，观察到使用GAG膜加替加德姆(TM)治疗的伤口的再上皮化显著高于单独使用替加德姆(TM)的伤口。虽然在伤口收缩或炎症反应方面没有发现明显的差异，但在第10天，HA和CS膜处理的创面显示出更多的纤维血管组织。GAG水凝胶膜提供了一个高度水化的细胞周围环境，在这个环境中，其他基质成分的组装、生长和分化因子的呈现以及细胞迁移都可以很容易地发生。(C)2002爱思唯尔科学有限公司。保留所有权利。
Chemically-crosslinked glycosaminoglycan (GAG) hydrogel films were prepared and evaluated as bio-interactive wound dressings. Hyaluronan (HA) and chondroitin sulfate (CS) were first converted to the adipic dihydrazide derivatives and then crosslinked with poly(ethylene glycol) propiondialdehyde to give a polymer network. The crosslinking occurred at neutral pH in minutes at room temperature to give clear, soft hydrogels. After gelation, a solvent-casting method was used to obtain a GAG hydrogel film. A mouse model was used to evaluate the efficacy of these GAG films in facilitating wound healing. Full-thickness wounds were created on the dorsal side of Balb/c mice and were dressed with a GAG film plus Tegaderm(TM) or Tegaderm(TM) alone. A significant increase in re-epithelialization was observed on day 5 (p < 0.001) and day 7) (p< 0.05) for wounds treated with a GAG film plus Tegaderm(TM) versus those treated with Tegaderm(TM) alone. While no significant differences in wound contraction or inflammatory response were found, wounds treated with either HA or CS films showed more fibro-vascular tissue by day 10. The GAG hydrogel films provide a highly hydrated, peri-cellular environment in which assembly of other matrix components, presentation of growth and differentiation factors, and cell migration can readily occur. (C) 2002 Elsevier Science Ltd. All rights reserved.