Microgeographic structure of Anopheles gambiae in western Kenya based on mtDNA and microsatellite loci
Microgeographic structure of Anopheles gambiae in western Kenya based on mtDNA and microsatellite loci
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DOI：
10.1046/j.1365-294x.1997.00177.x
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发表时间：
1997-03-01
影响因子：
4.9
通讯作者：
Collins, FH
中科院分区：
文献类型：
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作者：
Lehmann, T;Besansky, NJ;Collins, FH
利用5个微卫星位点的长度变异和648-nt mtDNA片段的序列变异研究了肯尼亚西部冈比亚按蚊的种群遗传结构。从村庄的房屋中收集蚊子，跨越长达50公里的距离。回答了以下问题：（i）室内蚊子的遗传相关性是否比室内蚊子的遗传相关性更高？(ii)在这些地理尺度上发生了何种程度的遗传分化？(iii)两种类型的遗传标记获得的结果有多一致？在家庭水平上，没有分化检测到的F-ST和R（ST），和带共享指数测试显示没有显着关联的等位基因在基因座之间。同样地，基于mtDNA单倍型的家庭亲属关系指数甚至低于合并样本。因此，室内蚊子在遗传上更相关的假设被否定了。在不断增加的地理尺度上，微卫星等位基因分布在所有群体样本中相似，并且F-ST或R（ST）未检测到基因库的细分。同样，估计的单倍型分歧的mtDNA之间的人口并不高于人口内的估计，和mtDNA为基础的F-ST值没有显着差异为零。线粒体DNA序列变异与微卫星位点的匹配结果（而在所有位点均观察到高遗传变异）表明这种模式代表了整个基因组。与A的一个德莫相关的最小面积。因此，肯尼亚西部的冈比亚直径大于50公里。
The population genetic structure of the Anopheles gambiae in western Kenya was studied using length variation at five microsatellite loci and sequence variation in a 648-nt mtDNA fragment. Mosquitoes were collected from houses in villages spanning up to 50 km distance. The following questions were answered, (i) Are mosquitoes in a house more related genetically to each other than mosquitoes between houses? (ii) What degree of genetic differentiation occurs on these geographical scales? (iii) How consistent are the results obtained with both types of genetic markers? At the house level, no differentiation was detected by F-ST and R(ST), and the band sharing index test revealed no significant associations of alleles across loci. Likewise, indices of kinship based on mtDNA haplotypes in houses were even lower than in the pooled sample. Therefore, the hypothesis that mosquitoes in a house are more related genetically was rejected. At increasing geographical scales, microsatellite allele distributions were similar among all population samples and no subdivision of the gene pool was detected by F-ST or R(ST). Likewise, estimates of haplotype divergence of mtDNA between populations were not higher than the within population estimates, and mtDNA-based F-ST values were not significantly different from zero. That sequence variation in mtDNA provided matching results with microsatellite loci (while high genetic variation was observed in all loci), suggested that this pattern represents the whole genome. The minimum area associated with a deme of A. gambiae in western Kenya is therefore larger than 50 km in diameter.