Modulation of Cortical Motor Evoked Potential After Stroke During Electrical Stimulation of the Lateral Cerebellar Nucleus.
Modulation of Cortical Motor Evoked Potential After Stroke During Electrical Stimulation of the Lateral Cerebellar Nucleus.
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DOI：
10.1016/j.brs.2015.06.020
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发表时间：
2015-11
影响因子：
7.7
通讯作者：
Machado AG
中科院分区：
文献类型：
--
作者：
Park HJ;Furmaga H;Cooperrider J;Gale JT;Baker KB;Machado AG
针对小脑外侧核 (LCN) 起源的齿状丘脑皮质 (DTC) 通路的深部脑刺激 (DBS) 已被证明可以增强皮质缺血啮齿动物模型的运动恢复。 LCN DBS 还产生正常大脑运动皮层兴奋性的频率特定变化，以运动诱发电位 (MEP) 幅度为索引。目的 研究啮齿动物缺血模型中皮质中风对皮质运动兴奋性的影响，并测量 LCN DBS 对缺血后兴奋性的影响（作为刺激参数的函数）。成年 Sprague-Dawley 大鼠被分为两组：幼稚组和中风组，通过多次单侧内皮素-1 注射诱导皮质缺血。所有动物都在受影响半球对面的 LCN 中植入双极电极。使用皮质内微刺激 (ICMS) 技术从受影响的半球引出 MEP。检查了多个 LCN DBS 参数，包括 20、30、50 和 100 Hz 的等时刺激以及新颖的突发刺激模式。相对于未接受治疗的动物 (n=12)，中风 (n=10) 动物中 ICMS 诱发的 MEP 减少。然而，两组 LCN DBS 均表现出频率依赖性的皮质兴奋性增强。在初治组中，LCN DBS 使 MEP 增加 22-58%，而在中风组中，与 OFF DBS 条件相比，MEP 增加 9-41%。 DTC 通路的激活会增加幼年动物和中风后动物的皮质兴奋性。这些效应可能至少部分是中风后动物与慢性 LCN DBS 相关的功能重组和治疗益处的基础。
Deep brain stimulation (DBS) targeting the dentato-thalamo-cortical (DTC) pathway at its origin in the lateral cerebellar nucleus (LCN) has been shown to enhance motor recovery in a rodent model of cortical ischemia. LCN DBS also yielded frequency specific changes in motor cortex excitability in the normal brain, indexed by motor evoked potential (MEP) amplitude. To investigate the effect of cortical stroke on cortical motor excitability in a rodent ischemia model and to measure the effects of LCN DBS on post-ischemia excitability as a function of stimulation parameters. Adult Sprague-Dawley rats were divided into two groups: naïve and stroke, with cortical ischemia induced through multiple, unilateral endothelin-1 injections. All animals were implanted with a bipolar electrode in the LCN opposite the affected hemisphere. MEPs were elicited from the affected hemisphere using intracortical microstimulation (ICMS) techniques. Multiple LCN DBS parameters were examined, including isochronal stimulation at 20, 30, 50, and 100 Hz as well as a novel burst stimulation pattern. ICMS-evoked MEPs were reduced in stroke (n=10) relative to naïve (n=12) animals. However, both groups showed frequency-dependent augmentation of cortical excitability in response to LCN DBS. In the naïve group, LCN DBS increased MEPs by 22–58%, while in the stroke group, MEPs were enhanced by 9–41% compared to OFF DBS conditions. Activation of the DTC pathway increases cortical excitability in both naïve and post-stroke animals. These effects may underlie, at least partially, functional reorganization and therapeutic benefits associated with chronic LCN DBS in post-stroke animals.