Effect of surface condition to temperature distribution in living tissue during cryopreservation
Effect of surface condition to temperature distribution in living tissue during cryopreservation
复制标题
表面条件对冷冻保存过程中活体组织温度分布的影响
DOI：
10.1088/1757-899x/278/1/012124
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
You Sugimoto and Hiyu Sasaki
中科院分区：
文献类型：
--
作者：
Masakazu Nozawa;Sho Hatakeyama;You Sugimoto and Hiyu Sasaki
当通过覆盖不锈钢网改变测试样品的表面状况时，测量模拟活组织的温度分布，以提高低温保存期间的冷却速率。琼脂用作模拟活组织，并填充在供试品内。测量了直接浸入液氮中的网状物的瞬态温度变化。通过使用T型热电偶测量样品表面和样品内部的温度。结果表明，在常压下，样品表面的温度比饱和液氮中的温度略有升高。通过比较有无网片时试样表面温度的变化，发现有网片时试样的表面温度低于无网片时试样的表面温度。另一方面，位于样品中心的温度的时间序列变化不随网格的存在或不存在而改变。据认为，所用样品的中心离表面太深，不能响应样品表面上的沸腾状态。
The temperature distribution of the simulated living tissue is measured for the improvement of the cooling rate during cryopreservation when the surface condition of the test sample is changed by covering the stainless steel mesh. Agar is used as a simulated living tissue and is filled inside the test sample. The variation of the transient temperature with mesh by the directly immersion in the liquid nitrogen is measured. The temperatures on the sample surface and the inside of the sample are measured by use of type T thermocouples. It is confirmed that on the sample surface there is the slightly temperature increase than that in the saturated liquid nitrogen at the atmospheric pressure. It is found by the comparison of the degree of superheat with or without the mesh that the surface temperature of the test sample with the mesh is lower than that without the mesh. On the other hand, the time series variations of the temperature located in the center of the sample does not change with or without the mesh. It is considered that the center of the sample used is too deep from the surface to respond to the boiling state on the sample surface.