A novel putative helicase produced in early murine lymphocytes
A novel putative helicase produced in early murine lymphocytes
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DOI：
10.1016/0378-1119(95)00843-8
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发表时间：
1996-03-09
期刊：
影响因子：
3.5
通讯作者：
Muegge, K
中科院分区：
文献类型：
--
作者：
Jarvis, CD;Geiman, T;Muegge, K
DNA解旋酶（Hel）在DNA链分离的许多过程中起作用，包括复制、修复、重组和转录。在未成熟淋巴细胞中发生的受体基因重排也可能由Hel介导。我们在此报道从小鼠胎儿胸腺组织中克隆了一种新的假定的Hel，它含有7个保守的Hel结构域，属于DNA Hel的DEGH亚类。我们将编码基因命名为lsh（淋巴特异性Hel），因为该基因在早期胸腺细胞中表达，但根据Northern分析，在心脏、肝脏、肺、肌肉、大脑或肾脏中不表达。脾细胞在激活后表达lsh。T和b细胞系在未成熟和成熟阶段均表达lsh。为了研究淋巴细胞形成的最早阶段，研究了小鼠胚胎组织；妊娠第12天卵黄囊中未检测到LSH，但妊娠第15天胎儿肝脏和胸腺中有高水平表达。
DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination and transcription. Rearrangement of receptor genes, which occurs in immature lymphocytes, could also be mediated by Hel. We report here the cloning from murine fetal thymus tissue of a novel putative Hel containing seven conserved Hel domains and belonging to the DEGH subclass of DNA Hel. We term the encoding gene lsh (lymphoid-specific Hel), since the gene is expressed in early thymocytes, but not in heart, liver, lung, muscle, brain or kidney, as judged by Northern analysis. Spleen cells expressed lsh following activation. T- and B-cell lines, at both the immature and mature stage, expressed lsh. To examine the earliest stages of lymphopoiesis, mouse embryonic tissues were examined; lsh was not detected in the yolk sac of day 12 of gestation, but was expressed in fetal liver and at high levels in fetal thymus at day 15 of gestation.