A chromosome-scale genome assembly of Antheraea pernyi (Saturniidae, Lepidoptera)
A chromosome-scale genome assembly of Antheraea pernyi (Saturniidae, Lepidoptera)
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柞蚕（Saturniidae，鳞翅目）染色体规模基因组组装
DOI：
10.1111/1755-0998.13199
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发表时间：
2020-08-20
影响因子：
7.7
通讯作者：
Wu, Xiangwei
中科院分区：
文献类型：
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作者：
Duan, Jianping;Li, Ying;Wu, Xiangwei
柞蚕是一种半驯化鳞翅目昆虫，对丝绸工业、人类健康和生态旅游具有重要价值。由于其经济影响和发育特性，它是研究蚕科超级科分化的理想模型。然而，对a的核型进化和功能基因组学的研究。基因资源的匮乏限制了植物的生长。本研究采用PacBio测序技术和染色体结构捕获技术组装了第一个高质量的ya。来自单个雄性个体的pernygenome。基因组长720.67 Mb，有49条染色体和一个13.77 Mb的支架N50。重复序列约为441.75 Mb，占基因组的60.74%。基因组包含21431个蛋白质编码基因，其中85.22%被功能注释。比较基因组学分析表明。松树从它的共同祖先中分化而来。与3030万年前的Yamamai相似，染色体分裂导致了染色体数量的增加。这项工作组装的基因组不仅有助于未来的研究。同时也有助于鳞翅目比较基因组学分析的进展。
Antheraea pernyiis a semi-domesticated lepidopteran insect species valuable to the silk industry, human health, and ecological tourism. Owing to its economic influence and developmental properties, it serves as an ideal model for investigating divergence of the Bombycoidea super family. However, studies on the karyotype evolution and functional genomics ofA. pernyiare limited by scarce genomic resource. Here, we applied PacBio sequencing and chromosome structure capture technique to assemble the first high-qualityA. pernyigenome from a single male individual. The genome is 720.67 Mb long with 49 chromosomes and a 13.77-Mb scaffold N50. Approximately 441.75 Mb, accounting for 60.74% of the genome, was identified as repeats. The genome comprises 21,431 protein-coding genes, 85.22% of which were functionally annotated. Comparative genomics analysis suggested thatA. pernyidiverged from its common ancestor withA. yamamai similar to 30.3 million years ago, and that chromosome fission contributed to the increased chromosome number. The genome assembled in this work will not only facilitate future research onA. pernyiand related species but also help to progress comparative genomics analyses in Lepidoptera.