Pseudogenes for human U2 small nuclear RNA do not have a fixed site of 3' truncation.
Pseudogenes for human U2 small nuclear RNA do not have a fixed site of 3' truncation.
复制标题
人 U2 小核 RNA 的假基因没有固定的 3 截短位点。
DOI：
10.1093/nar/12.3.1463
复制
发表时间：
1984
影响因子：
14.9
通讯作者：
Weiner,AM
中科院分区：
文献类型：
--
作者：
VanArsdell,SW;Weiner,AM
我们提供了与以前报道的U2.13假基因非常相似的另外5个人U2假基因的序列[货车Aredellet.al.（1981）Cell 26，11-17]。所有六个U2假基因都保留了成熟U2 snRNA序列的5′端，并且所有六个基因的侧翼都有几乎完美的正向重复序列，其序列不同，长度范围为16至21个碱基对。6个U2假基因的3端在33 - 82位之间的5个不同位点被截短，在两种情况下，假基因的3′端与下游直接重复序列重叠5或6个碱基对。这六个U2假基因的结构与四个人U3假基因的结构形成对比[Bernstrinetal.（1983）Cell 32，461-472，所有这些都在位置69或70处被相同地截短，并且似乎衍生自U3 snRNA的自引发的74个碱基的逆转录物。U2和U3假基因的比较表明了它们产生的模型，其中假基因的3′端总是相对于初始cDNA模板被截短。
We present the sequences of five additional human U2 pseudogenes which are very similar to the U2.l3 pseudogene reported previously [Van Aredellet.al. (1981) Cell26, 11–17]. All six U2 pseudogenes preserve the 5′ end of the mature U2 snRNA sequence, and all six are flanked by nearly perfect direct repeats that differ in sequence and range in length from 16 to 21 base pairs. The 3 ends of the six U2 pseudogenes are truncated at five different sites between position 33 and 82, and in two cases the 3′ end of the pseudogene overlaps the downstream direct repeat by 5 or 6 base pairs. The structure of these six U2 pseudogenes contrasts with that of four human U3 pseudogenes [Bernstrinetal. (1983) Cell32, 461–472 all of which are identically truncated at position 69 or 70, and appear to be derived from a self-primed 74 base reverse transcript of U3 snRNA. Comparison of the U2 and U3 pseudogenes suggests a model for their generation in which the 3′ end of the pseudogene is always truncated relative to the initial cDNA template.