QTL Identification and Fine Mapping for Seed Storability in Rice (Oryza sativa L.)
QTL Identification and Fine Mapping for Seed Storability in Rice (Oryza sativa L.)
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DOI：
10.1007/s10681-017-1913-5
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发表时间：
2017-06-01
期刊：
影响因子：
1.9
通讯作者：
Zhang, H. S.
中科院分区：
文献类型：
--
作者：
Li, C. S.;Shao, G. S.;Zhang, H. S.
水稻种子耐贮性是种子质量的重要指标，选育耐贮性强的品种是杂交水稻种子生产的重点。目前，水稻种子耐贮性的遗传和生理机制尚不清楚。本研究以粳稻品种CJ 06和籼稻品种TN 1杂交的双单倍体群体为材料，分析了影响水稻种子在自然贮藏和人工老化过程中发芽率和脂肪酸含量的数量性状基因座（QTL）。在9条染色体上共检测到19个QTL，其中GP QTL 10个，FA QTL 9个，表型变异范围为2.1%~ 22.7%。此外，GP和FA分别存在6对和3对上位性相互作用。此外，还将发芽率QTL qGP-9定位在STS标记P6和P8之间的92.8 kb区间内，包含15个开放阅读框。这些结果为深入了解水稻种子耐贮性的遗传机制提供了重要信息，也为选育高耐贮性水稻新品种提供了理论依据。
Rice seed storability is an important characteristic of seed quality so that the cultivars with strong seed storability are expected in the production of hybrid seeds. Presently, little is known about the genetic and physiological mechanisms controlling rice seed storability. In this study, a double haploid population derived from the cross between a japonica cultivar CJ06 and an indica cultivar TN1 was used to identify the quantitative trait loci (QTLs) for seed germination percentage (GP) and fatty acid content (FA) during natural storage or artificial aging. A total of 19 QTLs, including ten QTLs for GP and nine QTLs for FA, were identified on nine chromosomes with the phenotypic variations ranged from 2.1 to 22.7%. Besides, six and three pairs of epistatic interactions were identified for GP and FA, respectively. Moreover, qGP-9, a QTL for germination percentage, was delimited in an interval of 92.8 kb between two STS markers P6 and P8, which contains 15 putative open reading frames. These results provide important information for understanding the genetic mechanisms on rice seed storability, and will be useful for breeding new rice varieties with high seed storability.