NaCl RESPONSIVE TASTE CELLS IN THE MOUSE FUNGIFORM TASTE BUDS
NaCl RESPONSIVE TASTE CELLS IN THE MOUSE FUNGIFORM TASTE BUDS
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DOI：
10.1016/j.neuroscience.2008.12.052
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发表时间：
2009-03-17
期刊：
影响因子：
3.3
通讯作者：
Ninomiya, Y.
中科院分区：
文献类型：
--
作者：
Yoshida, R.;Horio, N.;Ninomiya, Y.
以往的研究表明，啮齿类动物的鼓索（CT）神经纤维对氯化钠的反应可以分为两组：阿米洛利敏感（AS）和不敏感（Al）的上皮钠通道（ENaC）阻断剂的敏感性。AS纤维被证明是专门响应NaCl，而铝纤维广泛响应各种电解质，包括NaCl。这些数据表明，在味觉细胞的盐味转导可能是由至少两个不同的系统：AS和Al的。为了进一步解决这个问题，我们研究了NaCl，KCl和HCl的反应和阿米洛利的敏感性小鼠菌状乳头味蕾细胞的CT神经支配。结果表明，56个NaCl敏感细胞可分为两组：25个细胞（44%）对NaCl有较窄的反应，阿米洛利可抑制其反应（AS细胞），而其余31个细胞（类似于56%）不仅对NaCl有反应，而且对KCl和/或HCl有反应，并且没有显示阿米洛利对NaCl反应的抑制（Al细胞）。阿米洛利应用于味觉细胞的基底外侧没有影响在AS和Al细胞的NaCl反应。单细胞逆转录-聚合酶链反应（RT-PCR）实验表明，ENaC亚基mRNA的表达在一个子集的AS细胞。这些结果表明，小鼠菌状味蕾是由AS和Al细胞，可以传递不同的味觉信息，其相应类型的CT纤维，和顶端ENaCs可能参与了NaCl的AS细胞的反应。(C)2009年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Previous studies have demonstrated that rodents' chorda tympani (CT) nerve fibers responding to NaCl can be classified according to their sensitivities to the epithelial sodium channel (ENaC) blocker amiloride into two groups: amiloride-sensitive (AS) and -insensitive (Al). The AS fibers were shown to respond specifically to NaCl, whereas Al fibers broadly respond to various electrolytes, including NaCl. These data suggest that salt taste transduction in taste cells may be composed of at least two different systems; AS and Al ones. To further address this issue, we investigated the responses to NaCl, KCl and HCl and the amiloride sensitivity of mouse fungiform papilla taste bud cells which are innervated by the CT nerve. Comparable with the CT data, the results indicated that 56 NaCl-responsive cells tested were classified into two groups; 25 cells (similar to 44%) narrowly responded to NaCl and their NaCl response were inhibited by amiloride (AS cells), whereas the remaining 31 cells (similar to 56%) responded not only to NaCl, but to KCl and/or HCl and showed no amiloride inhibition of NaCl responses (Al cells). Amiloride applied to the basolateral side of taste cells had no effect on NaCl responses in the AS and Al cells. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments indicated that ENaC subunit mRNA was expressed in a subset of AS cells. These findings suggest that the mouse fungiform taste bud is composed of AS and Al cells that can transmit taste information differently to their corresponding types of CT fibers, and apical ENaCs may be involved in the NaCl responses of AS cells. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.