The human platelet as a reproducible and sensitive cell for the detection and assay of platelet-activating factor.
The human platelet as a reproducible and sensitive cell for the detection and assay of platelet-activating factor.
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人血小板作为一种可重复且敏感的细胞，用于检测和测定血小板激活因子。
DOI：
10.1016/0003-2697(92)90142-t
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发表时间：
1992
影响因子：
2.9
通讯作者：
Hanahan,DJ
中科院分区：
文献类型：
--
作者：
Ekholm,J;Tatsumi,Y;Nouchi,T;Hanahan,DJ
一种新的程序，用于制备人血小板一贯敏感的血小板活化因子（PAF）在低纳摩尔范围内已经开发出来。这种方法成功的关键是在分离阶段添加腺苷，提供了稳定细胞的良好恢复，并在聚集测定中包含ADP，提供了对PAF的增加的灵敏度。在ADP存在或不存在的情况下，氚标记的PAF与这些血小板的结合曲线的检查揭示了Kd值的显着差异，但在特定的结合位点的数量没有。描述了对人血小板的反应性和稳定性具有影响的其它试剂，关于其与PAF的相互作用。
A new procedure for the preparation of human platelets consistently sensitive to platelet-activating factor (PAF) in the low nanomolar range has been developed. Key to the success of this approach was the addition of adenosine during the isolation phase, providing an excellent recovery of stable cells, and the inclusion of ADP in the aggregation assay, providing increased sensitivity to PAF. Examination of the binding profile of tritium-labeled PAF to these platelets in the presence or absence of ADP revealed significant difference in the Kdvalues but not in the number of specific binding sites. Other reagents having an influence on the reactivity and stability of the human platelets, as regards its interaction with PAF, are described.