Electrospray deposition as a method to fabricate functionally active protein films
Electrospray deposition as a method to fabricate functionally active protein films
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DOI：
10.1021/ac9808775
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发表时间：
1999-04-01
影响因子：
7.4
通讯作者：
Morozova, TY
中科院分区：
文献类型：
--
作者：
Morozov, VN;Morozova, TY
电喷雾电离是蛋白质和其他生物聚合物的常规分析方法。建议将电喷雾产品沉积在导电电极上作为一种制备功能活性蛋白质膜的方法。以恢复电喷雾碱性磷酸酶(AP)的比水解力为探针，在电喷雾沉积(ESD)中保存蛋白质的完整性。结果表明，蛋白质在静电作用下的失活与所用电压和电流高度相关。湿度和溶液中保护物质的存在也会影响这一过程。在低电流、低湿度、含二糖的条件下进行ESD，可完全保持酶的活性。
Electrospray ionization is a routine method in MS analysis of proteins and other biopolymers. Deposition of the electrospray products onto a conductive electrode is suggested here as a means to manufacture functionally active protein films. Recovery of the specific hydrolytic activity of the electrosprayed alkaline phosphatase (AP) was used as a probe for preservation of protein intactness in the electrospray deposition (ESD). It was shown that protein inactivation upon ESD is highly dependent on voltage and current used. Humidity and the presence of protective substances in solution also affect the process. Complete preservation of the enzyme activity was observed when the ESD was performed at low current and humidity in the presence of disaccharides.