Genetic analysis of drought tolerance in maize by molecular markers. II. Plant height and flowering
Genetic analysis of drought tolerance in maize by molecular markers. II. Plant height and flowering
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DOI：
10.1007/s001220051234
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发表时间：
1999-07-01
影响因子：
5.4
通讯作者：
Frova, C
中科院分区：
文献类型：
--
作者：
Sari-Gorla, M;Krajewski, P;Frova, C
干旱是一个严重的农业问题，也是造成作物产量损失的重要因素之一。在温带地区生长的玉米中，干旱胁迫发生在开花期之前和期间：因此，该物种对水分胁迫的耐受性主要取决于开花时或开花后不久发生的事件。本研究的目的是：（1）确定与植物发育和开花相关的耐旱性因子所在的染色体区域，（2）将这些区域与控制这些性状的QTL进行比较，以获得玉米对水分胁迫响应的遗传和生理基础的间接信息。为此，我们进行了雄性和雌性开花时间，开花吐丝间隔（ASI），株高和分子标记之间的连锁分析。本研究以B73和H99自交F1代获得的142个玉米重组自交系为材料，在充分供水和水分胁迫两种环境条件下，利用RFLP、SSR和AFLP标记对153个位点进行了基因型分析。连锁分析结果表明，在控制和胁迫条件下检测到的雄株开花时间和株高的QTL大部分相同。与此相反，对于女性开花时间和ASI不同的QTL似乎在控制条件下或胁迫下表达。所有赋予耐旱性的QTL都位于与控制该性状本身的因子的图谱位置不同的染色体位置。这表明，植物的耐受性，在其不同的组成部分，是不是归因于控制性状的有利等位基因组合的存在，而是基于生理特性不直接与控制的字符。
Drought is a serious agronomic problem, and one of the most important factors contributing to crop yield loss. In maize grown in temperate areas, drought stress occurs just before and during the flowering period: consequently, tolerance to water stress in this species is largely determined by events that occur at or shortly after flowering. The purposes of our investigation were: (1)to identify the chromosomal regions where factors conferring drought tolerance for traits related to plant development and flowering are located and (2) to compare these regions with those carrying QTLs controlling these traits, in order to get indirect information on the genetic and physiological basis of maize response to water stress. To this aim, we performed a linkage analysis between the expression of male and female flowering time, anthesis-silking interval (ASI), plant height and molecular markers. The experiment was carried out under two environmental conditions, well-watered and water-stressed, on a maize population of 142 recombinant inbred lines obtained by selfing the F-1 between lines B73 and H99 and genotyped by RFLP, microsatellites (SSR) and AFLP markers, for a total of 153 loci. Linkage analysis revealed that, for male flowering time and plant height, most of the QTLs detected were the same under control and stress conditions. In contrast, with respect to female flowering time and ASI diverse QTLs appeared to be expressed either under control conditions or under stress. All of the QTLs conferring tolerance to drought were located in a different chromosome position as compared to the map position of the factors controlling the trait per se. This suggests that plant tolerance, in its different components, is not attributable to the presence of favourable allelic combinations controlling the trait but is based on physiological characteristics not directly associated with the control of the character.