A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells
A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells
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DOI：
10.1016/j.canlet.2008.09.036
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发表时间：
2009-03-08
期刊：
影响因子：
9.7
通讯作者：
Yoshikawa, Toshikazu
中科院分区：
文献类型：
--
作者：
Gen, Yasuyuki;Yasui, Kohichiroh;Yoshikawa, Toshikazu
RhoA是小gtpase Rho家族的一员，指导肌动蛋白细胞骨架的组织，并参与调节细胞形状和运动。其活性受p190-B RhoGAP （gtpase -激活蛋白）负调控。我们利用高密度寡核苷酸芯片研究了人肝癌和食管鳞癌细胞系的DNA拷贝数畸变，在染色体14q12区发现了一个新的扩增位点。我们确定了ARHGAP5（编码p190-B RhoGAP的基因）可能是14q12扩增的靶标，我们的结果表明p190-B RhoGAP通过负向调节Huh-7肝癌细胞中的RhoA活性来促进细胞的扩散和迁移。2008爱思唯尔爱尔兰有限公司版权所有。
RhoA, a member Of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells. (C) 2008 Elsevier Ireland Ltd. All rights reserved.