Characterization of Anthocyanidin Synthase (ANS) Gene and anthocyanidin in rare medicinal plant-Saussurea medusa
Characterization of Anthocyanidin Synthase (ANS) Gene and anthocyanidin in rare medicinal plant-Saussurea medusa
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珍稀药用植物——水母雪莲中花青素合酶（ANS）基因及花青素的特性研究
DOI：
10.1007/s11240-007-9211-x
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发表时间：
2007-04-01
影响因子：
3
通讯作者：
Zhao, Dexiu
中科院分区：
文献类型：
--
作者：
Cheng, Liqin;Xu, Yanjun;Zhao, Dexiu
花青素合酶（ANS，无色花青素加氧酶）是一种 2-氧戊二酸铁依赖性加氧酶，催化花青素类黄酮类生物合成的倒数第二步，从无色无色花青素到有色花青素。在这里，我们报道了从传统中药用植物雪莲红色愈伤组织系中提取的 cDNA 文库中的花青素合酶基因 (SmANS1) 的克隆和表征。 SmANS1 在氨基酸序列上与 2-酮戊二酸依赖性加氧酶家族的成员表现出显着的相似性。推导的SmANS1蛋白与中国红花和非洲菊杂交种的同源性分别为83%和79%。对SmANS1转录本的逆转录PCR(RT-PCR)分析表明，SmANS1在红细胞系和幼苗的细胞培养物中大量表达，在黄细胞系的细胞培养物中表达较弱，但在根中不表达。此外，使用高效液相色谱/质谱（HPLC-ESI-MS）和串联质谱（MS-MS）研究了水母雪莲细胞培养物的花青素组成。主要花青素被鉴定为 3-O-葡萄糖苷花青素衍生物，次要峰被鉴定为天竺葵素衍生物。
Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzes the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. Here, we report the cloning and characterization of an anthocyanidin Synthase gene (SmANS1) from a cDNA library derived from a red callus line ofSaussurea medusa, a traditional Chinese medicinal plant. SmANS1 exhibits significant similarities in amino acid sequence with members of a family of 2-oxoglutarate-dependent oxygenases. The deduced SmANS1 protein shared 83% and 79% identity with that ofCallistephus chinensisandGerbera hybrids, respectively. Reverse transcription-PCR (RT-PCR) analyses ofSmANS1transcripts showed that theSmANS1was abundantly expressed in the cell cultures of red cell line and seedlings, and weakly expressed in the counterparts of yellow cell line, but was not expressed in the roots. Further more, the anthocyanins composition of cell cultures fromSaussurea medusawas investigated using high-performance liquid chromatography/mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (MS-MS). Major anthocyanins were identified as 3-O-glucosides cyanidin derivatives and minor peaks were identified as pelargonidin derivatives.