New insights in the composition of extracellular vesicles from pancreatic cancer cells: implications for biomarkers and functions.
New insights in the composition of extracellular vesicles from pancreatic cancer cells: implications for biomarkers and functions.
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DOI：
10.1186/s12953-014-0050-5
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发表时间：
2014
期刊：
影响因子：
2
通讯作者：
Schwarte-Waldhoff I
中科院分区：
文献类型：
--
作者：
Klein-Scory S;Tehrani MM;Eilert-Micus C;Adamczyk KA;Wojtalewicz N;Schnölzer M;Hahn SA;Schmiegel W;Schwarte-Waldhoff I
胰腺癌的发生与促结缔组织反应和免疫逃逸等特征性改变有关，这些改变是由细胞间的通讯机制和细胞的微环境介导的。在这些过程中，发布的组件的整体是重要的决定因素。尤其是胰腺癌细胞释放的胞外小泡在细胞通讯、调节细胞生长和免疫反应中起着重要作用。在这里，我们给出了来自胰腺癌细胞的亲和纯化的胞外囊泡的蛋白质组学描述，与分泌组相比，分泌组被定义为胰腺癌细胞释放的全部蛋白质。蛋白质组学数据提供了数百种蛋白质的全面目录，比较揭示了胰腺癌细胞来源的胞外小泡的特殊蛋白质组组成。蛋白质组成的功能分析表明，膜蛋白、糖蛋白、小的GTP结合蛋白和一组进一步的异质性蛋白质富含在囊泡中，而来自蛋白酶体和核糖体的蛋白以及代谢酶不是囊泡的组成部分。此外，在致癌过程中起作用的蛋白质和细胞外基质(ECM)或细胞-细胞相互作用的调节器是亲和纯化的胞外囊泡的组成部分。这些数据通过数百种以前没有被描述为胰腺癌细胞释放的囊泡成分的蛋白质加深了对细胞外囊泡成分的了解。来自胰腺癌细胞的胞外小泡显示了与其他小泡共有的蛋白质以及细胞类型特定的蛋白质，表明了候选的生物标记物，并暗示了在癌细胞间质相互作用中的功能作用。本文的在线版本(doi：10.1186/s12953-01400505)包含补充材料，授权用户可以使用。
Pancreatic cancer development is associated with characteristic alterations like desmoplastic reaction and immune escape which are mediated by the cell-cell communication mechanism and by the microenvironment of the cells. The whole of released components are important determinants in these processes. Especially the extracellular vesicles released by pancreatic cancer cells play a role in cell communication and modulate cell growth and immune responses. Here, we present the proteomic description of affinity purified extracellular vesicles from pancreatic tumour cells, compared to the secretome, defined as the whole of the proteins released by pancreatic cancer cells. The proteomic data provide comprehensive catalogues of hundreds of proteins, and the comparison reveals a special proteomic composition of pancreatic cancer cell derived extracellular vesicles. The functional analysis of the protein composition displayed that membrane proteins, glycoproteins, small GTP binding proteins and a further, heterogeneous group of proteins are enriched in vesicles, whereas proteins derived from proteasomes and ribosomes, as well as metabolic enzymes, are not components of the vesicles. Furthermore proteins playing a role in carcinogenesis and modulators of the extracellular matrix (ECM) or cell-cell interactions are components of affinity purified extracellular vesicles. The data deepen the knowledge of extracellular vesicle composition by hundreds of proteins that have not been previously described as vesicle components released by pancreatic cancer cells. Extracellular vesicles derived from pancreatic cancer cells show common proteins shared with other vesicles as well as cell type specific proteins indicating biomarker candidates and suggesting functional roles in cancer cell stroma interactions. The online version of this article (doi:10.1186/s12953-014-0050-5) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Clayton, Aled;Al-Taei, Saly;Tabi, Zsuzsanna
通讯作者：
Tabi, Zsuzsanna
影响因子：
4.4
作者：
Chelala, Claude;Hahn, Stephan A.;Crnogorac-Jurcevic, Tatjana
通讯作者：
Crnogorac-Jurcevic, Tatjana
影响因子：
4
作者：
Colombo, Marina;Moita, Catarina;Raposo, Graca
通讯作者：
Raposo, Graca
影响因子：
14.9
作者：
Chen J;Bardes EE;Aronow BJ;Jegga AG
通讯作者：
Jegga AG
DOI：
10.1038/nrm3117
发表时间：
2011-06
期刊：
Nature reviews. Molecular cell biology
影响因子：
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作者：
通讯作者：
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