Induction of interferon-γ by Taenia crassiceps glycans and Lewis sugars in naive BALB/c spleen and peritoneal exudate cells
Induction of interferon-γ by Taenia crassiceps glycans and Lewis sugars in naive BALB/c spleen and peritoneal exudate cells
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DOI：
10.1016/j.molimm.2006.08.019
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发表时间：
2007-03-01
影响因子：
3.6
通讯作者：
Shahin, Allen
中科院分区：
文献类型：
--
作者：
Dissanayake, Senarath;Shahin, Allen
Helminth parasites are known to alter host immune responses and the responsible molecules area potential source of biological immunoadjuvants. Previously, we have reported strong Th-2 type immunomodulatory properties of Taenia crassiceps glycans. In this study, we report interferon-gamma (IFN-gamma) stimulatory activity of fractionated Taenia glycans and Lewis sugars with comparable glycan composition. Our data show that Taenia Glycans and Lewis X pentasaccharide are potent stimulators of the Th-1 type cytokine IFN-gamma. We postulate that the terminal beta-(1-4)-galactose residue in Lewis X is associated with IFN-gamma stimulation from naive BALB/c mouse spleen and peritoneal exudate cells. Antibodies to toll-like receptors (TLRs) inhibited the Lewis X-induced IFN-gamma secretion. Lewis X up-regulated the expression of NF-kappa B p65 from naive spleen cells and IFN-gamma transcription in peritoneal exudate cells. These data demonstrate the ability of Lewis type helminth glycans to modulate host responses in a Th-1 direction via NF-kappa B p65, IFN-gamma and macrophage TLRs. (c) 2006 Elsevier Ltd. All rights reserved.
