Genome-Wide Screening of mRNA Expression in Leprosy Patients.
Genome-Wide Screening of mRNA Expression in Leprosy Patients.
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DOI：
10.3389/fgene.2015.00334
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发表时间：
2015
影响因子：
3.7
通讯作者：
Soares CT
中科院分区：
文献类型：
--
作者：
Belone Ade F;Rosa PS;Trombone AP;Fachin LR;Guidella CC;Ura S;Barreto JA;Pinilla MG;de Carvalho AF;Carraro DM;Soares FA;Soares CT
麻风是一种由麻风分枝杆菌引起的传染病，全世界有数百万人患有麻风病。然而，对其分子病理生理机制却知之甚少。在这项研究中，利用DNA芯片微阵列对麻风皮肤病变进行了全面的人类mRNA评估，包括疾病的整个谱及其反应状态。以66例麻风皮损(10TT、10BT、10BB、10BL、4LL、14R1、10R2)和9例健康人皮肤活检作为对照(CC)，年龄06~83岁，男48例，女29例。这项评估确定了1580个差异表达的≥[折叠变化(Fc)mRNA2.0，p≤0.05]在疾病皮损和健康对照中。其中一些基因在所有类型的疾病(CD2、CD27、it1、FA2H、FAM26F、GZMB、MMP9、SLAMF7、UBD)中都有表达，另一些基因是反应型的(类型1反应：GPNMB、IL1B、MICAL2、FOXQ1；类型2反应：AKR1B10、FAM180B、FOXQ1、NNMT、NR1D1、PTX3、TNFRSF25)。在文献中，这些mRNAs与许多病理生理过程和信号通路有关，并存在于大量疾病中。这些mRNAs的作用可以在开发新的麻风病诊断标记物和治疗靶点的背景下进行研究。
Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1580 differentially expressed mRNAs [Fold Change (FC) ≥ 2.0, p ≤ 0.05] in diseased lesions vs. healthy controls. Some of these genes were observed in all forms of the disease (CD2, CD27, chit1, FA2H, FAM26F, GZMB, MMP9, SLAMF7, UBD) and others were exclusive to reactional forms (Type “1” reaction: GPNMB, IL1B, MICAL2, FOXQ1; Type “2” reaction: AKR1B10, FAM180B, FOXQ1, NNMT, NR1D1, PTX3, TNFRSF25). In literature, these mRNAs have been associated with numerous pathophysiological processes and signaling pathways and are present in a large number of diseases. The role of these mRNAs maybe studied in the context of developing new diagnostic markers and therapeutic targets for leprosy.